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Literature summary extracted from

  • Lee, C.; Zhong, R.; Ye, Z.
    Biochemical characterization of xylan xylosyltransferases involved in wood formation in poplar (2012), Plant Signal. Behav., 7, 332-337.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.24 expressed in tobacco BY2 cells Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.24 5.27
-
 xylotetraose co-substrate: UDP-D-xylose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication Populus trichocarpa
2.4.2.24 5.27
-
 UDP-D-xylose co-substrate: xylotetraose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication Populus trichocarpa
2.4.2.24 5.9
-
 xylotetraose co-substrate: UDP-D-xylose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication Populus trichocarpa
2.4.2.24 5.9
-
 UDP-D-xylose co-substrate: xylotetraose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication Populus trichocarpa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.24 microsome
-
 Populus trichocarpa
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.24 Populus trichocarpa B9HH87
-
-
2.4.2.24 Populus trichocarpa B9IJ10
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.24 UDP-D-xylose + xylotetraose microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. XylT activity can not use the xylose monomer as an acceptor. Xylosyl residues are readily transferred onto xylooligomers ranging from 2 to 6 residues, resulting in the production of xylooligosaccharides with up to 11 xylosyl residues Populus trichocarpa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.24 PtrGT43B
-
 Populus trichocarpa
2.4.2.24 PtrGT43C
-
 Populus trichocarpa

General Information

EC Number General Information Comment Organism
2.4.2.24 malfunction microsomes from transgenic tobacco BY2 cells coexpressing PtrGT43B and PtrGT43C exhibit a high XylT activity capable of generating beta-(1,4)-linked xylooligosaccharides, whereas little XylT activity is detected in microsomes with expression of PtrGT43B or PtrGT43C alone Populus trichocarpa
2.4.2.24 physiological function PtrGT43B and PtrGT43C, are xylan XylTs involved in wood formation Populus trichocarpa