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Literature summary extracted from

  • Suzuki, T.; Honda, Y.; Funatsuki, W.; Nakatsuka, K.
    Purification and characterization of flavonol 3-glucosidase, and its activity during ripening in tartary buckwheat seeds (2002), Plant Sci., 163, 417-423.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B31 0.115
-
 rutin pH 5.0, 37°C, isoenzyme f3gI Fagopyrum tataricum
3.2.1.B31 0.125
-
 rutin pH 5.0, 37°C, isoenzyme f3gII Fagopyrum tataricum
3.2.1.B31 1.05
-
 isoquercitrin pH 5.0, 37°C, isoenzyme f3gI Fagopyrum tataricum
3.2.1.B31 1.15
-
 isoquercitrin pH 5.0, 37°C, isoenzyme f3gII Fagopyrum tataricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B31 57400
-
 x * 57400, isoenzyme f3gII, SDS-PAGE Fagopyrum tataricum
3.2.1.B31 58200
-
 x * 58200, isoenzyme f3gI, SDS-PAGE Fagopyrum tataricum
3.2.1.B31 89000
-
 isoenzyme f3gI and isoenzyme f4gII, gel filtration Fagopyrum tataricum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B31 Fagopyrum tataricum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B31
-
 Fagopyrum tataricum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.B31 seed testa Fagopyrum tataricum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B31 135.7
-
 pH 5.0, 37°C Fagopyrum tataricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B31 isoquercitrin + H2O i.e. quercetin 3-O-beta-D-glucoside, Vmax value for isoquercitrin is one-tenth of that for rutin Fagopyrum tataricum quercetin + beta-D-glucose
-
 ?
3.2.1.B31 rutin + H2O i.e. quercetin 3-O-beta-D-rutenoside Fagopyrum tataricum quercetin + rutinose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B31 ? x * 57400, isoenzyme f3gII, SDS-PAGE Fagopyrum tataricum
3.2.1.B31 ? x * 58200, isoenzyme f3gI, SDS-PAGE Fagopyrum tataricum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B31 flavonol 3-glucosidase
-
 Fagopyrum tataricum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B31 37
-
 assay at Fagopyrum tataricum
3.2.1.B31 40
-
-
 Fagopyrum tataricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B31 5
-
-
 Fagopyrum tataricum