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Literature summary extracted from

  • Post, J.; van Deenen, N.; Fricke, J.; Kowalski, N.; Wurbs, D.; Schaller, H.; Eisenreich, W.; Huber, C.; Twyman, R.M.; Pruefer, D.; Gronover, C.S.
    Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of Taraxacum brevicorniculatum (2012), Plant Physiol., 158, 1406-1417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.20 expression of isozymes TbCPT1, TbCPT2, and TbCPT3 associated with rubber particles in transgenic Taraxacum brevicorniculatum plants Taraxacum brevicorniculatum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.20 additional information CPT-RNAi plants show a significant reduction in rubber biosynthesis and a corresponding 50% increase in the levels of triterpenes and the main storage carbohydrate, inulin Taraxacum brevicorniculatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.20 polycis-polyprenyl diphosphate + isopentenyl diphosphate Taraxacum kok-saghyz
-
 diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit
-
 ?
2.5.1.20 polycis-polyprenyl diphosphate + isopentenyl diphosphate Taraxacum brevicorniculatum
-
 diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.20 Taraxacum brevicorniculatum
-
 isozymes TbCPT1, TbCPT2, and TbCPT3
-
2.5.1.20 Taraxacum kok-saghyz
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.20 latex the milky cytoplasm of specialized cells known as laticifers Taraxacum kok-saghyz
-
2.5.1.20 latex the milky cytoplasm of specialized cells known as laticifers Taraxacum brevicorniculatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.20 polycis-polyprenyl diphosphate + isopentenyl diphosphate
-
 Taraxacum kok-saghyz diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit
-
 ?
2.5.1.20 polycis-polyprenyl diphosphate + isopentenyl diphosphate
-
 Taraxacum brevicorniculatum diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.20 chain-elongating enzyme cis-prenyltransferase
-
 Taraxacum kok-saghyz
2.5.1.20 chain-elongating enzyme cis-prenyltransferase
-
 Taraxacum brevicorniculatum
2.5.1.20 CPT
-
 Taraxacum kok-saghyz
2.5.1.20 CPT
-
 Taraxacum brevicorniculatum
2.5.1.20 laticifer-specific rubber-forming enzyme
-
 Taraxacum kok-saghyz
2.5.1.20 laticifer-specific rubber-forming enzyme
-
 Taraxacum brevicorniculatum

Expression

EC Number Organism Comment Expression
2.5.1.20 Taraxacum brevicorniculatum CPT expression is reduced by laticifer-specific RNA interference, CPT-RNAi down

General Information

EC Number General Information Comment Organism
2.5.1.20 malfunction CPT-RNAi plants indicate a significant reduction in rubber biosynthesis and a corresponding 50% increase in the levels of triterpenes and inulin, the primary storage carbohydrate compared to wild-type plants. The laticifers in CPT-RNAi plants contained fewer and smaller rubber particles than wild-type laticifers. And a decrease is noted in the activity of hydroxymethylglutaryl-CoA reductase, a key enzyme in the mevalonate pathway, reflecting homeostatic control of the isopentenyl diphosphate pool, CPT RNAi silencing phenotypes, overview Taraxacum brevicorniculatum
2.5.1.20 physiological function the species produce large amounts of high-quality natural rubber in its latex. This high-molecular mass biopolymer consists mainly of poly(cis-1,4-isoprene) and is deposited in rubber particles by particle-bound enzymes that carry out the condensation of isopentenyl diphosphate units. Large polymer formation is carried out by the chain-elongating enzyme cis-prenyltransferase, CPT Taraxacum kok-saghyz
2.5.1.20 physiological function the species produce large amounts of high-quality natural rubber in its latex. This high-molecular mass biopolymer consists mainly of poly(cis-1,4-isoprene) and is deposited in rubber particles by particle-bound enzymes that carry out the condensation of isopentenyl diphosphate units. Large polymer formation is carried out by the chain-elongating enzyme cis-prenyltransferase, CPT Taraxacum brevicorniculatum