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Literature summary extracted from

  • Lyi, S.M.; Heller, L.I.; Rutzke, M.; Welch, R.M.; Kochian, L.V.; Li, L.
    Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli (2005), Plant Physiol., 138, 409-420.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.280 BoSMT overexpression in Escherichia coli strain MTD123 Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.280 37900
-
 calculated from protein sequence Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.280 S-adenosyl-L-methionine + L-selenocysteine Brassica oleracea selenate-treated plants synthesize significantly more Se-methyl-L-selenocysteine S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.280 Brassica oleracea Q4VNK0 var. italica
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.280 floret
-
 Brassica oleracea
-
2.1.1.280 leaf
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.280 0.001
-
 with 10 mM DL-homocysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
2.1.1.280 0.0011
-
 with 10 mM L-cysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
2.1.1.280 0.0018
-
 with 10 mM DL-cysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
2.1.1.280 0.0024
-
 with 0.5 mM L-selenocysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea
2.1.1.280 0.0051
-
 with 0.5 mM DL-selenocysteine as methyl acceptor and [methyl-14C]adenosylmethionine as the methyl donor, pH 7.0 and 25°C Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.280 additional information expression of BoSMT significantly enhances Se tolerance with an increased level of total Se accumulation in Escherichia coli Brassica oleracea ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + DL-cysteine
-
 Brassica oleracea ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + DL-homocysteine
-
 Brassica oleracea ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + DL-selenocysteine highest preference as methyl acceptor Brassica oleracea ?
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-cysteine
-
 Brassica oleracea S-adenosyl-L-homocysteine + S-methyl-L-cysteine
-
 ?
2.1.1.280 S-adenosyl-L-methionine + L-selenocysteine selenate-treated plants synthesize significantly more Se-methyl-L-selenocysteine Brassica oleracea S-adenosyl-L-homocysteine + Se-methyl-L-selenocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.280 BoSMT
-
 Brassica oleracea
2.1.1.280 SMT
-
 Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.280 7
-
-
 Brassica oleracea

Expression

EC Number Organism Comment Expression
2.1.1.280 Brassica oleracea concentrations higher than 40 microM show a negative effect on BoSMT mRNA accumulation. At very high selenate concentrations e.g. 100 microM, the expression of BoSMT mRNA substantially reduced down
2.1.1.280 Brassica oleracea high sulfate levels in growth medium significantly reduce accumulation of BoSMT transcripts in plants down
2.1.1.280 Brassica oleracea treating Brassica oleracea plants with selenate results in a rapid accumulation of BoSMT mRNA and reaches a maximum expression at selenate levels between 20 and 40 microM. This is in contrast to 40 microM selenite, which causes only a slight accumulation of the transcript up

General Information

EC Number General Information Comment Organism
2.1.1.280 metabolism SMT is the key enzyme for Se-methylselenocysteine synthesis Brassica oleracea
2.1.1.280 physiological function expression of BoSMT significantly enhances Se tolerance, high levels of Se accumulate in Broccoli plants exposed to selenate, but addition of high levels of 1 or 10 mM sulfate shows a strong inhibitory effect on Se accumulation Brassica oleracea