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Literature summary extracted from

  • Borejsza-Wysocki, W.; Hrazdina, G.
    Aromatic polyketide synthases (purification, characterization, and antibody development to benzalacetone synthase from raspberry fruits) (1996), Plant Physiol., 110, 791-799.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.212 0.001
-
 malonyl-CoA pH 8.0, 30°C Rubus idaeus
2.3.1.212 0.003
-
 4-coumaroyl-CoA pH 8.0, 30°C Rubus idaeus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.212 41500
-
 2 * 41500, SDS-PAGE Rubus idaeus
2.3.1.212 83000
-
 gel filtration Rubus idaeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O Rubus idaeus
-
 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O Rubus idaeus Royalty
-
 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.212 Rubus idaeus
-
-
-
2.3.1.212 Rubus idaeus Royalty
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.212 native enzyme 172fold from fruits by ammonium sulfate fractionation, gel filtration, hydrophobic interaction and anion exchange chromatography, and chromatofocusing Rubus idaeus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.212 fruit
-
 Rubus idaeus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O high activity with 4-coumaroyl-CoA Rubus idaeus 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 4-coumaroyl-CoA + malonyl-CoA + H2O high activity with 4-coumaroyl-CoA Rubus idaeus Royalty 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
2.3.1.212 5-hydroxyferuloyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 5-hydroxyferuloyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 caffeoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 caffeoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 cinnamoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 cinnamoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 feruloyl-CoA + malonyl-CoA + H2O feruloyl-CoA is the best substrate showing 3fold higher activity than 4-coumaroyl-CoA Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
2.3.1.212 sinapoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.212 dimer 2 * 41500, SDS-PAGE Rubus idaeus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.212 30
-
 assay at Rubus idaeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.212 8
-
-
 Rubus idaeus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.212 Rubus idaeus chromatofocusing
-
 5.3

Expression

EC Number Organism Comment Expression
2.3.1.212 Rubus idaeus rapid induction of the enzyme in cell suspension cultures upon addition of yeast extract up

General Information

EC Number General Information Comment Organism
2.3.1.212 physiological function 4-hydroxybenzalacetone, i.e. 4-hydroxyphenylbutan-2-one, the characteristic aroma compound of raspberries, is synthesized from 4-coumaryl-CoA and malonyl-CoA in a two-step reaction sequence that is catalyzed by benzalacetone synthase and benzalacetone reductase. 4-Hydroxybenzalacetone inhibits mycelial growth of the raspberry pathogen Phytophthora fragariae var. rub Rubus idaeus