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Literature summary extracted from
- Fumarate and cytosolic pH as modulators of the synthesis or consumption of C4 organic acids through NADP-malic enzyme in Arabidopsis thaliana (2013), Plant Mol. Biol., 81, 297-307.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.40 | fumarate | fumarate activates the NADP-ME2 forward reaction by about 15fold at 0.05 mM NADP+, but only at about 1.5fold at 0.35 mM NADP+. The trans configuration of fumarate is crucial for the activating effect | Arabidopsis thaliana |  |
| 1.1.1.40 | succinate | activates at 10 mM | Arabidopsis thaliana | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.40 | expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.40 | R115A | site-directed mutagenesis of isozyme NADP-ME2, the mutant displays a marked inhibition in the presence of all the organic acids tested, also fumarate | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.40 | 2-oxoglutarate | inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
| 1.1.1.40 | Maleate | the cis isomer of fumarate, inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
| 1.1.1.40 | malonate | inhibition of isozyme NADP-ME2 | Arabidopsis thaliana | |
| 1.1.1.40 | additional information | no inhibition of isozyme NADP-ME2 by tartrate | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.40 | additional information | - |
additional information | kinetics of NADP+ and (S)-malate at different NADP+ and fumarate concentrations, overview | Arabidopsis thaliana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.40 | Mg2+ | required | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.40 | (S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.40 | Arabidopsis thaliana | - |
four malic isozymes, NADP-ME1, NADP-ME2, NADP-ME3, and NADP-ME4 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.40 | (S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.40 | NADP-dependent malic enzyme | - |
Arabidopsis thaliana |
| 1.1.1.40 | NADP-ME | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.40 | 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.40 | 7 | - |
reductive carboxylation of pyruvate | Arabidopsis thaliana |
| 1.1.1.40 | 7.5 | - |
oxidative decarboxylation of malate | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.40 | NADP+ | dependent on | Arabidopsis thaliana | |
| 1.1.1.40 | NADPH | dependent on | Arabidopsis thaliana | |
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