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Literature summary extracted from

  • Frydman, A.; Liberman, R.; Huhman, D.V.; Carmeli-Weissberg, M.; Sapir-Mir, M.; Ophir, R.; W Sumner, L.; Eyal, Y.
    The molecular and enzymatic basis of bitter/non-bitter flavor of citrus fruit: evolution of branch-forming rhamnosyltransferases under domestication (2013), Plant J., 73, 166-178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B53 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of 1,6RhaT in BY-2 cells Citrus reticulata
2.4.1.B53 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of 1,6RhaT in BY-2 cells Citrus clementina
2.4.1.B53 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of 1,6RhaT in BY-2 cells Citrus sinensis
2.4.1.236 1,2RhaT cloning and DNA and amino acid sequence determination and analysis, phylogenetic analysis, two native frameshift mutations render the enzyme from orange catalytically inactive Citrus sinensis
2.4.1.236 phylogenetic analysis, 1,2RhaT expressionin BY-2 cells Citrus maxima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B53 additional information Citrus reticulata the enzyme specifically catalyzes rhamnosylation of 7-O-glucosides of flavanones, flavones, flavonols, and anthocyanidins at position 6 of the glucose moiety ?
-
 ?
2.4.1.B53 additional information Citrus clementina the enzyme specifically catalyzes rhamnosylation of 7-O-glucosides of flavanones, flavones, flavonols, and anthocyanidins at position 6 of the glucose moiety ?
-
 ?
2.4.1.B53 additional information Citrus sinensis the enzyme specifically catalyzes rhamnosylation of 7-O-glucosides of flavanones, flavones, flavonols, and anthocyanidins at position 6 of the glucose moiety ?
-
 ?
2.4.1.B53 UDP-L-rhamnose + hesperetin 7-O-beta-D-glucoside Citrus reticulata
-
 UDP + hesperetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. hesperidin ?
2.4.1.B53 UDP-L-rhamnose + hesperetin 7-O-beta-D-glucoside Citrus clementina
-
 UDP + hesperetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. hesperidin ?
2.4.1.B53 UDP-L-rhamnose + hesperetin 7-O-beta-D-glucoside Citrus sinensis
-
 UDP + hesperetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. hesperidin ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside Citrus reticulata
-
 UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. narirutin ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside Citrus clementina
-
 UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. narirutin ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside Citrus sinensis
-
 UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. narirutin ?
2.4.1.236 additional information Citrus maxima the enzyyme specifically catalyzes rhamnosylation of flavanone-7-O-glucose and flavone-7-O-glucose substrates at position 2 of the glucose moiety ?
-
 ?
2.4.1.236 UDP-L-rhamnose + diosmetin 7-O-glucoside Citrus maxima a flavone 7-O-glucoside UDP + diosmetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neodiosmin, a bitter 7-O-neohesperidoside ?
2.4.1.236 UDP-L-rhamnose + hesperetin 7-O-glucoside Citrus maxima
-
 UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neohesperidin, a bitter 7-O-neohesperidoside ?
2.4.1.236 UDP-L-rhamnose + naringenin 7-O-glucoside Citrus maxima
-
 UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. naringin, a bitter 7-O-neohesperidoside ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B53 Citrus clementina
-
-
-
2.4.1.B53 Citrus reticulata
-
-
-
2.4.1.B53 Citrus sinensis A7ISD3
-
-
2.4.1.B53 no activity in Citrus maxima
-
-
-
2.4.1.236 Citrus maxima
-
-
-
2.4.1.236 Citrus sinensis
-
-
-
2.4.1.236 Citrus sinensis valencia
-
-
-
2.4.1.236 no activity in Citrus reticulata
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.B53 fruit high in young fruits Citrus reticulata
-
2.4.1.B53 fruit high in young fruits Citrus clementina
-
2.4.1.B53 fruit high in young fruits Citrus sinensis
-
2.4.1.B53 leaf high in young leaves Citrus reticulata
-
2.4.1.B53 leaf high in young leaves Citrus clementina
-
2.4.1.B53 leaf high in young leaves Citrus sinensis
-
2.4.1.236 fruit
-
 Citrus maxima
-
2.4.1.236 leaf
-
 Citrus maxima
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B53 additional information the enzyme specifically catalyzes rhamnosylation of 7-O-glucosides of flavanones, flavones, flavonols, and anthocyanidins at position 6 of the glucose moiety Citrus reticulata ?
-
 ?
2.4.1.B53 additional information the enzyme specifically catalyzes rhamnosylation of 7-O-glucosides of flavanones, flavones, flavonols, and anthocyanidins at position 6 of the glucose moiety Citrus clementina ?
-
 ?
2.4.1.B53 additional information the enzyme specifically catalyzes rhamnosylation of 7-O-glucosides of flavanones, flavones, flavonols, and anthocyanidins at position 6 of the glucose moiety Citrus sinensis ?
-
 ?
2.4.1.B53 additional information Cs1,6RhaT catalyzes branched-chain rhamnosylation of both 7-O-beta-D-glucoside and 3-O-beta-D-glucoside substrates Citrus reticulata ?
-
 ?
2.4.1.B53 additional information Cs1,6RhaT catalyzes branched-chain rhamnosylation of both 7-O-beta-D-glucoside and 3-O-beta-D-glucoside substrates Citrus clementina ?
-
 ?
2.4.1.B53 additional information Cs1,6RhaT is a substrate-promiscuous flavonoid branchforming rhamnnosyltransferase, Cs1,6RhaT catalyzes branched-chain rhamnosylation of both 7-O-beta-D-glucoside and 3-O-beta-D-glucoside substrates Citrus sinensis ?
-
 ?
2.4.1.B53 UDP-L-rhamnose + cyanidin 3-O-beta-D-glucoside a anthocyanidin 7-O-glucoside Citrus reticulata UDP + cyanidin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + cyanidin 3-O-beta-D-glucoside a anthocyanidin 7-O-glucoside Citrus clementina UDP + cyanidin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + cyanidin 3-O-beta-D-glucoside a anthocyanidin 7-O-glucoside Citrus sinensis UDP + cyanidin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + diosmetin 7-O-beta-D-glucoside a flavone 7-O-glucoside Citrus reticulata UDP + diosmetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. diosmin ?
2.4.1.B53 UDP-L-rhamnose + diosmetin 7-O-beta-D-glucoside a flavone 7-O-glucoside Citrus clementina UDP + diosmetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. diosmin ?
2.4.1.B53 UDP-L-rhamnose + diosmetin 7-O-beta-D-glucoside a flavone 7-O-glucoside Citrus sinensis UDP + diosmetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. diosmin ?
2.4.1.B53 UDP-L-rhamnose + hesperetin 7-O-beta-D-glucoside
-
 Citrus reticulata UDP + hesperetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. hesperidin ?
2.4.1.B53 UDP-L-rhamnose + hesperetin 7-O-beta-D-glucoside
-
 Citrus clementina UDP + hesperetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. hesperidin ?
2.4.1.B53 UDP-L-rhamnose + hesperetin 7-O-beta-D-glucoside
-
 Citrus sinensis UDP + hesperetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. hesperidin ?
2.4.1.B53 UDP-L-rhamnose + kaempferol 3-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus reticulata UDP + kaempferol 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + kaempferol 3-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus clementina UDP + kaempferol 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + kaempferol 3-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus sinensis UDP + kaempferol 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + kaempferol 7-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus reticulata UDP + kaempferol 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + kaempferol 7-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus clementina UDP + kaempferol 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + kaempferol 7-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus sinensis UDP + kaempferol 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus reticulata UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. narirutin ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus clementina UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. narirutin ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus sinensis UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. narirutin ?
2.4.1.B53 UDP-L-rhamnose + peonidin 3-O-beta-D-glucoside a anthocyanidin 7-O-glucoside Citrus reticulata UDP + peonidin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + peonidin 3-O-beta-D-glucoside a anthocyanidin 7-O-glucoside Citrus clementina UDP + peonidin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + peonidin 3-O-beta-D-glucoside a anthocyanidin 7-O-glucoside Citrus sinensis UDP + peonidin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus reticulata UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus clementina UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus sinensis UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 7-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus reticulata UDP + quercetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 7-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus clementina UDP + quercetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 7-O-beta-D-glucoside a flavonol 7-O-glucoside Citrus sinensis UDP + quercetin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.236 additional information the enzyyme specifically catalyzes rhamnosylation of flavanone-7-O-glucose and flavone-7-O-glucose substrates at position 2 of the glucose moiety Citrus maxima ?
-
 ?
2.4.1.236 additional information the enzyme also catalyzes the rhamnosylation of diosmetin 7-O-glucoside at position 2 of the glucose moiety to give neodiosmin, but Cm1,2RhaT is specific for flavonoid-7-O-glucosides and utilizes no flavonoid-3-O-glucosides Citrus maxima ?
-
 ?
2.4.1.236 UDP-L-rhamnose + diosmetin 7-O-glucoside a flavone 7-O-glucoside Citrus maxima UDP + diosmetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neodiosmin, a bitter 7-O-neohesperidoside ?
2.4.1.236 UDP-L-rhamnose + hesperetin 7-O-glucoside
-
 Citrus maxima UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neohesperidin, a bitter 7-O-neohesperidoside ?
2.4.1.236 UDP-L-rhamnose + hesperetin 7-O-glucoside a flavanone 7-O-glucoside Citrus maxima UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neohesperidin, a bitter 7-O-neohesperidoside ?
2.4.1.236 UDP-L-rhamnose + naringenin 7-O-glucoside
-
 Citrus maxima UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. naringin, a bitter 7-O-neohesperidoside ?
2.4.1.236 UDP-L-rhamnose + naringenin 7-O-glucoside a flavanone 7-O-glucoside Citrus maxima UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. naringin, a bitter 7-O-neohesperidoside ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B53 1,6-rhamnosyltransferase
-
 Citrus reticulata
2.4.1.B53 1,6-rhamnosyltransferase
-
 Citrus clementina
2.4.1.B53 1,6-rhamnosyltransferase
-
 Citrus sinensis
2.4.1.B53 1,6RhaT
-
 Citrus reticulata
2.4.1.B53 1,6RhaT
-
 Citrus clementina
2.4.1.B53 1,6RhaT
-
 Citrus sinensis
2.4.1.B53 branch-forming flavanone-7-O-glucose 1,6-rhamnosyltransferase
-
 Citrus reticulata
2.4.1.B53 branch-forming flavanone-7-O-glucose 1,6-rhamnosyltransferase
-
 Citrus clementina
2.4.1.B53 branch-forming flavanone-7-O-glucose 1,6-rhamnosyltransferase
-
 Citrus sinensis
2.4.1.B53 flavanone-7-O-glucose-1,6-rhamnosyltransferase
-
 Citrus reticulata
2.4.1.B53 flavanone-7-O-glucose-1,6-rhamnosyltransferase
-
 Citrus clementina
2.4.1.B53 flavanone-7-O-glucose-1,6-rhamnosyltransferase
-
 Citrus sinensis
2.4.1.236 1,2-rhamnosyltransferase
-
 Citrus sinensis
2.4.1.236 1,2-rhamnosyltransferase
-
 Citrus maxima
2.4.1.236 1,2RhaT
-
 Citrus sinensis
2.4.1.236 1,2RhaT
-
 Citrus maxima
2.4.1.236 flavanone-7-O-glucose-1,2-rhamnosyltransferase
-
 Citrus sinensis
2.4.1.236 flavanone-7-O-glucose-1,2-rhamnosyltransferase
-
 Citrus maxima

General Information

EC Number General Information Comment Organism
2.4.1.B53 metabolism the enzyme is involved in the biosynthesis of flavonoid branched-chain glycosides in Citrus, overview. 1,6RhaT is a branch-forming enzyme directing biosynthesis of the tasteless flavanone rutinoside compounds in non-bitter Citrus species Citrus reticulata
2.4.1.B53 metabolism the enzyme is involved in the biosynthesis of flavonoid branched-chain glycosides in Citrus, overview. 1,6RhaT is a branch-forming enzyme directing biosynthesis of the tasteless flavanone rutinoside compounds in non-bitter Citrus species Citrus clementina
2.4.1.B53 metabolism the enzyme is involved in the biosynthesis of flavonoid branched-chain glycosides in Citrus, overview. 1,6RhaT is a branch-forming enzyme directing biosynthesis of the tasteless flavanone rutinoside compounds in non-bitter Citrus species Citrus sinensis
2.4.1.236 metabolism the enzyme is involved in the biosynthesis of flavonoid branched-chain glycosides in citrus, overview. 1,2RhaT is the key branch-forming enzyme directing biosynthesis of the bitter flavanone neohesperidoside compounds in bitter Citrus species Citrus maxima