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Literature summary extracted from

  • Yonekura-Sakakibara, K.; Fukushima, A.; Nakabayashi, R.; Hanada, K.; Matsuda, F.; Sugawara, S.; Inoue, E.; Kuromori, T.; Ito, T.; Shinozaki, K.; Wangwattana, B.; Yamazaki, M.; Saito, K.
    Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana (2012), Plant J., 69, 154-167.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.51 gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli Arabidopsis thaliana
2.4.2.51 recombinantly expressed as a His-tagged fusion protein Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.51 additional information construction of transposon insertion mutants of UGT79B1 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.51 UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside Arabidopsis thaliana
-
 UDP + cyanidin 3-O-beta-D-sambubioside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.51 Arabidopsis thaliana Q9LVW3
-
-
2.4.2.51 Arabidopsis thaliana Q9LVW3 gene ugt79b1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.51 recombinant His-tagged enzyme from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.51 additional information enzyme converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1-2)glucoside. Enzyme recognizes 3-O-glucosylated anthocyanidins/flavonols and uridine diphosphate (UDP)-xylose Arabidopsis thaliana ?
-
 ?
2.4.2.51 additional information UGT79B1 recognizes 3-O-glucosylated anthocyanidins/flavonols and UDP-xylose, but not 3,5-O-diglucosylated anthocyanidins, substrate specificity, overview. No activity with cyanidin 3-O-rhamnoside, cyanidin 3,5-O-diglucoside, cyanidin 3-O-6''-O-4-coumaroylglucoside-5-O-glucoside, cyanidin 3-O-6''-O-p-coumaroylglucoside, kaempferol 3-O-rhanoside, and kaempferol 3-O-glucoside-7-O-rhamnoside. The enzyme is absolutely specific for UDP-xylose as donar substrate, no activity with UDP-arabinose, UDP-galactose, UDP-glucose, UDP-glucuronic acid, and UDP-rhamnose Arabidopsis thaliana ?
-
 ?
2.4.2.51 UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
 Arabidopsis thaliana UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
 ?
2.4.2.51 UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + cyanidin 3-O-beta-D-sambubioside
-
 ?
2.4.2.51 UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + delphinidin 3-O-beta-D-sambubioside
-
 ?
2.4.2.51 UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
 Arabidopsis thaliana UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside
-
 ?
2.4.2.51 UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside best substrate Arabidopsis thaliana UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside
-
 ?
2.4.2.51 UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + pelargonidin 3-O-beta-D-sambubioside
-
 ?
2.4.2.51 UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + quercetin 3-O-beta-D-sambubioside
-
 ?
2.4.2.51 UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside)
-
 Arabidopsis thaliana UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
 ?
2.4.2.51 UDP-D-xylose + cyanidin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
 ?
2.4.2.51 UDP-D-xylose + delphinidin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
 ?
2.4.2.51 UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
 Arabidopsis thaliana UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
 ?
2.4.2.51 UDP-D-xylose + kaempferol 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
 ?
2.4.2.51 UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
 ?
2.4.2.51 UDP-D-xylose + quercetin 3-O-beta-D-glucoside
-
 Arabidopsis thaliana UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.51 anthocyanin 3-O-glucoside: 2''-O-xylosyltransferase
-
 Arabidopsis thaliana
2.4.2.51 UGT79B1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.51 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.51 6.5
-
-
 Arabidopsis thaliana
2.4.2.51 7.5
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.4.2.51 evolution flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides Arabidopsis thaliana
2.4.2.51 malfunction anthocyanin is drastically reduced in ugt79b1 knockout mutants Arabidopsis thaliana