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Literature summary extracted from

  • Dudareva, N.; D'Auria, J.C.; Nam, K.H.; Raguso, R.A.; Pichersky, E.
    Acetyl-CoA:benzylalcohol acetyltransferase - an enzyme involved in floral scent production in Clarkia breweri (1998), Plant J., 14, 297-304.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.224 expression in Escherichia coli Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.224 48200
-
x * 48200, calculated from sequence Clarkia breweri
2.3.1.224 58000
-
1 * 58000, SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.224 acetyl-CoA + benzyl alcohol Clarkia breweri
-
CoA + benzyl acetate
-
?
2.3.1.224 acetyl-CoA + cinnamyl alcohol Clarkia breweri
-
CoA + cinnamyl acetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.224 Clarkia breweri O64988
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.224
-
Clarkia breweri

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.224 acetyl-CoA + benzyl alcohol = CoA + benzyl acetate (1) Clarkia breweri
2.3.1.224 acetyl-CoA + cinnamyl alcohol = CoA + cinnamyl acetate (2) Clarkia breweri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.224 additional information no BEAT mRNA is detected in leaves Clarkia breweri
-
2.3.1.224 petal petal contain the majority of BEAT transcripts Clarkia breweri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.224 acetyl-CoA + 2-(naphthalen-2-yl)ethanol at 9% of the activity compared to benzyl alcohol, plant purified enzyme, at 28% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme) Clarkia breweri CoA + 2-(naphthalen-2-yl)ethyl acetate
-
?
2.3.1.224 acetyl-CoA + 2-phenylethanol at 33% of the activity compared to benzyl alcohol, plant purified enzyme, at 57% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme) Clarkia breweri CoA + 2-phenylethylacetate
-
?
2.3.1.224 acetyl-CoA + benzyl alcohol
-
Clarkia breweri CoA + benzyl acetate
-
?
2.3.1.224 acetyl-CoA + cinnamyl alcohol
-
Clarkia breweri CoA + cinnamyl acetate
-
?
2.3.1.224 acetyl-CoA + cinnamyl alcohol at 88% of the activity compared to benzyl alcohol (plant purified enzyme), at 97% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme) Clarkia breweri CoA + cinnamyl acetate
-
?
2.3.1.224 additional information no activity with: 2-hydroxybenzylalcohol, 3-hydroxybenzylalcohol, 4-hydroxybenzylalcohol and linalool Clarkia breweri ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.224 ? 1 * 58000, SDS-PAGE Clarkia breweri
2.3.1.224 ? x * 48200, calculated from sequence Clarkia breweri

Synonyms

EC Number Synonyms Comment Organism
2.3.1.224 BEAT
-
Clarkia breweri

Expression

EC Number Organism Comment Expression
2.3.1.224 Clarkia breweri three days after anthesis, mRNA levels steeply declines down
2.3.1.224 Clarkia breweri the levels of BEAT mRNA in the petals increases as the bud matures, and peaks at anthesis up

General Information

EC Number General Information Comment Organism
2.3.1.224 physiological function the enzyme is important for floral scent production Clarkia breweri