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Literature summary extracted from

  • Varbanova, M.; Yamaguchi, S.; Yang, Y.; McKelvey, K.; Hanada, A.; Borochov, R.; Yu, F.; Jikumaru, Y.; Ross, J.; Cortes, D.; Ma, C.J.; Noel, J.P.; Mander, L.; Shulaev, V.; Kamiya, Y.; Rodermel, S.; Weiss, D.; Pichersky, E.
    Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2 (2007), Plant Cell, 19, 32-45.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.275 NH4+ 5 mM, 4fold activation Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.275 expression in Escherichia coli, expression in Arabidopsis under the control of the 35S cauliflower mosaic virus promoter Arabidopsis thaliana
2.1.1.276 expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.275 Cu2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
2.1.1.275 Fe2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
2.1.1.275 Fe3+ 5 mM, more than 90% inhibition Arabidopsis thaliana
2.1.1.275 Zn2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
2.1.1.276 Cu2+ 5 mM, more than 90% inhibition Arabidopsis thaliana
2.1.1.276 Fe3+ 5 mM, more than 90% inhibition Arabidopsis thaliana
2.1.1.276 Zn2+ 5 mM, more than 90% inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.275 0.0054
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.275 0.0158
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.276 0.0019
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.276 0.0059
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.275 K+ 5 mM, 3fold activation Arabidopsis thaliana
2.1.1.276 additional information not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.275 40000
-
 x * 40000, SDS-PAGE Arabidopsis thaliana
2.1.1.275 41500
-
 x * 41500, calculated from sequence Arabidopsis thaliana
2.1.1.276 43300
-
 x * 43300, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.275 S-adenosyl-L-methionine + gibberellin A9 Arabidopsis thaliana methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature S-adenosyl-L-homocysteine + methyl gibberellin A9
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A4 Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + methyl gibberellin A4
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.275 Arabidopsis thaliana F4JUY5
-
-
2.1.1.276 Arabidopsis thaliana Q5XF78
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.275
-
 Arabidopsis thaliana
2.1.1.276
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.275 seed GAMT1 expression is localized mostly to seed Arabidopsis thaliana
-
2.1.1.275 silique expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development Arabidopsis thaliana
-
2.1.1.276 seed
-
 Arabidopsis thaliana
-
2.1.1.276 silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.275 S-adenosyl-L-methionine + gibberellin 1 25% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A1
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A19 19% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A19
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A20 95% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A20
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A3 80% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A3
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A34 46% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A34
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A4 69% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A4
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A51 32% of the activity compared to methylation of gibberellin A9 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A51
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A9 methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A9
-
 ?
2.1.1.275 S-adenosyl-L-methionine + gibberellin A9 gibberellin A9 i.e. (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylic acid Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A9 methyl gibberellin A9 i.e. methyl (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylate ?
2.1.1.276 additional information no activity with gibberelin A12 and gibberelin A19 Arabidopsis thaliana ?
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A1 30% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A1
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A20 15% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A20
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A3 45% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A3
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A34 80% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A34
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A4
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A4
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A51 16% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A51
-
 ?
2.1.1.276 S-adenosyl-L-methionine + gibberellin A9 60% of the activity compared to methylation of gibberellin A4 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl gibberellin A9
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.275 ? x * 40000, SDS-PAGE Arabidopsis thaliana
2.1.1.275 ? x * 41500, calculated from sequence Arabidopsis thaliana
2.1.1.276 ? x * 43300, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.1.1.275 GAMT1
-
 Arabidopsis thaliana
2.1.1.276 GAMT2
-
 Arabidopsis thaliana
2.1.1.276 gibberellin A4 O-methyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.275 30
-
 assay at Arabidopsis thaliana
2.1.1.276 30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.275 0.01
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.275 0.026
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.276 0.0015
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.276 0.0018
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.275 7.5
-
-
 Arabidopsis thaliana
2.1.1.276 8
-
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.1.1.275 Arabidopsis thaliana expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development up
2.1.1.276 Arabidopsis thaliana expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development up

General Information

EC Number General Information Comment Organism
2.1.1.275 malfunction GAMT1 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol Arabidopsis thaliana
2.1.1.275 physiological function methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature Arabidopsis thaliana
2.1.1.276 malfunction GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol Arabidopsis thaliana
2.1.1.276 physiological function methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.275 1.6
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.275 1.85
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.276 0.2
-
 gibberellin a4 pH 8.0, 30°C Arabidopsis thaliana
2.1.1.276 0.9
-
 gibberellin a9 pH 8.0, 30°C Arabidopsis thaliana