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Literature summary extracted from

  • Zhang, Q.; Lee, J.; Pandurangan, S.; Clarke, M.; Pajak, A.; Marsolais, F.
    Characterization of Arabidopsis serine:glyoxylate aminotransferase, AGT1, as an asparagine aminotransferase (2013), Phytochemistry, 85, 30-35.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.45 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.45 0.59
-
 glycine cosubstrate glyoxylate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 0.87
-
 L-alanine cosubstrate glyoxylate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 0.99
-
 L-serine cosubstrate 3-hydroxypyruvate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 1.08
-
 L-alanine cosubstrate 3-hydroxypyruvate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 1.58
-
 glycine cosubstrate 3-hydroxypyruvate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 2.47
-
 L-serine cosubstrate glyoxylate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 2.82
-
 L-asparagine cosubstrate glyoxylate, pH 8.0, 30°C Arabidopsis thaliana
2.6.1.45 8.41
-
 L-asparagine cosubstrate 3-hydroxypyruvate, pH 8.0, 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.45 Arabidopsis thaliana Q56YA5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.45 glycine + 3-hydroxypyruvate
-
 Arabidopsis thaliana glyoxylate + L-serine
-
 r
2.6.1.45 glycine + pyruvate
-
 Arabidopsis thaliana glyoxylate + L-alanine
-
 r
2.6.1.45 L-alanine + 3-hydroxypyruvate
-
 Arabidopsis thaliana pyruvate + L-serine
-
 r
2.6.1.45 L-alanine + glyoxylate
-
 Arabidopsis thaliana pyruvate + glycine
-
 r
2.6.1.45 L-asparagine + glyoxylate
-
 Arabidopsis thaliana 2-oxosuccinamate + glycine
-
 r
2.6.1.45 L-asparagine + pyruvate
-
 Arabidopsis thaliana 2-oxosuccinamate + L-alanine
-
 r
2.6.1.45 L-serine + glyoxylate
-
 Arabidopsis thaliana 3-hydroxypyruvate + glycine
-
 r
2.6.1.45 L-serine + pyruvate
-
 Arabidopsis thaliana 3-hydroxypyruvate + L-alanine
-
 r

Expression

EC Number Organism Comment Expression
2.6.1.45 Arabidopsis thaliana In the roots of 10-day-old seedlings treated for 2 h with 20 mM Asn, the transcript levels are raised by 2fold. During this treatment, the concentration of Asn in root is raised by ca. 5fold up