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Literature summary extracted from

  • Kovinich, N.; Saleem, A.; Arnason, J.T.; Miki, B.
    Functional characterization of a UDP-glucose:flavonoid 3-O-beta-D-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.) (2010), Phytochemistry, 71, 1253-1263.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.91 expressed in Escherichia coli BL21(DE3) pLysS cells and in a Arabidopsis thaliana mutant (ugt78d2) deficient in anthocyanidin and flavonol 3-O-glucosyltransferase activity Glycine max
2.4.1.115 expressed in the Arabidopsis thaliana T-DNA mutant (ugt78d2) deficient in anthocyanidin and flavonol 3-O-beta-D-glucosyltransferase activity and in Escherichia coli BL21(DE3) pLysS cells Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.91 cyanidin the recombinant enzyme exhibits pronounced substrate inhibition by cyanidin at 0.1 mM Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 0.174
-
 kaempferol recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30°C Glycine max
2.4.1.115 0.174
-
 kaempferol recombinant enzyme, at pH 8.0 and 30°C Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 49240
-
 x * 49240, calculated from amino acid sequence Glycine max
2.4.1.91 49800
-
 x * 49800, estimated from SDS-PAGE Glycine max
2.4.1.115 49240
-
 x * 49240, calculated from amino acid sequence Glycine max
2.4.1.115 49800
-
 x * 49800, SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Glycine max D3Y5N8 cf. EC 2.4.1.115; cultivar Clark line
-
2.4.1.115 Glycine max D3Y5N8 cultivar Clark line
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91 Talon metal affinity column chromatography Glycine max
2.4.1.115 Talon metal affinity column chromatography, gel filtration Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 embryo
-
 Glycine max
-
2.4.1.91 flower bud
-
 Glycine max
-
2.4.1.91 leaf
-
 Glycine max
-
2.4.1.91 pod
-
 Glycine max
-
2.4.1.91 root
-
 Glycine max
-
2.4.1.91 seed coat
-
 Glycine max
-
2.4.1.91 stem
-
 Glycine max
-
2.4.1.115 embryo
-
 Glycine max
-
2.4.1.115 flower bud
-
 Glycine max
-
2.4.1.115 leaf
-
 Glycine max
-
2.4.1.115 pod
-
 Glycine max
-
2.4.1.115 root
-
 Glycine max
-
2.4.1.115 seed coat
-
 Glycine max
-
2.4.1.115 stem
-
 Glycine max
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.115 4°C or -20°C, 50% glycerol solution, 24 h, no significant loss of activity Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 additional information no activity with dihydroquercetin, genistin, genistein, daidzein, catechin, epicatechin, apigeninidin, flavonol 3-O-glucosides, anthocyanidin 3-O-glucosides and cyanidin 3-O-galactoside Glycine max ?
-
 ?
2.4.1.91 UDP-alpha-D-glucose + cyanidin
-
 Glycine max UDP + cyanidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + delphinidin best substrate Glycine max UDP + delphinidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin
-
 Glycine max UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + kaempferol
-
 Glycine max UDP + kaempferol 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + malvidin
-
 Glycine max UDP + malvidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + myricetin
-
 Glycine max UDP + myricetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + pelargonidin
-
 Glycine max UDP + pelargonidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + peonidin
-
 Glycine max UDP + peonidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + petunidin
-
 Glycine max UDP + petunidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + quercetin
-
 Glycine max UDP + quercetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-galactose + cyanidin galactose can be transferred from UDP-galactose to the 3-position of cyanidin with low specificity relative to the analogous glucosyltransferase reactions Glycine max ?
-
 ?
2.4.1.91 UDP-galactose + delphinidin galactose can be transferred from UDP-galactose to the 3-position of delphinidin with low specificity relative to the analogous glucosyltransferase reactions Glycine max ?
-
 ?
2.4.1.115 additional information no activity is observed for dihydroflavonol (dihydroquercetin), isoflavone (genistin, genistein and daidzein), (epi)-flavan-3-O-beta-Dl (catechin, epicatechin), 3-deoxyanthocyanidin (apigeninidin), flavonol conjugate (flavonol 3-O-beta-D-glucosides) and anthocyanins (anthocyanidin 3-O-beta-D-glucosides and cyanidin 3-O-beta-D-galactoside). Galactose can be transferred from UDP-galactose to the 3-position of cyanidin and delphinidin with low specificity relative to the analogous glucosyltransferase reactions Glycine max ?
-
 ?
2.4.1.115 UDP-glucose + cyanidin
-
 Glycine max UDP + cyanidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + delphinidin when acceptor substrates are supplied below the inhibitory concentration of cyaniding, the enzyme demonstrates highest activity towards the anthocyanidin delphinidin Glycine max UDP + delphinidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + isorhamnetin
-
 Glycine max UDP + isorhamnetin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + kaempferol when acceptor substrates are supplied above the inhibitory concentration of cyanidin, the enzyme demonstrates highest activity for flavonols (quercetin, myricetin, and kaempferol) Glycine max UDP + kaempferol-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + malvidin
-
 Glycine max UDP + malvidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + myricetin when acceptor substrates are supplied above the inhibitory concentration of cyanidin , the enzyme demonstrates highest activity for flavonols (quercetin, myricetin, and kaempferol) Glycine max UDP + myricetin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + pelargonidin
-
 Glycine max UDP + pelargonidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + peonidin
-
 Glycine max UDP + peonidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + petunidin
-
 Glycine max UDP + petunidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + quercetin when acceptor substrates are supplied above the inhibitory concentration of cyanidin , the enzyme demonstrates highest activity for flavonols (quercetin, myricetin, and kaempferol) Glycine max UDP + quercetin-3-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.91 ? x * 49240, calculated from amino acid sequence Glycine max
2.4.1.91 ? x * 49800, estimated from SDS-PAGE Glycine max
2.4.1.115 ? x * 49800, SDS-PAGE Glycine max
2.4.1.115 ? x * 49240, calculated from amino acid sequence Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.4.1.91 UDP-glucose:flavonoid 3-O-glucosyltransferase
-
 Glycine max
2.4.1.91 UGT78K1
-
 Glycine max
2.4.1.115 UDP-glucose:flavonoid 3-O-glucosyltransferase
-
 Glycine max
2.4.1.115 UF3GT
-
 Glycine max
2.4.1.115 UGT78K1
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.91 30
-
-
 Glycine max
2.4.1.115 30
-
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.91 1.25
-
 kaempferol recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30°C Glycine max
2.4.1.115 1.25
-
 kaempferol recombinant enzyme, at pH 8.0 and 30°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 8
-
 the recombinant enzyme exhibits a slight buffer preference for HEPES over Tris at pH 8.0 Glycine max
2.4.1.115 8
-
 the recombinant enzyme exhibits a slight buffer preference for HEPES over Tris buffer at pH 8.0 Glycine max

Expression

EC Number Organism Comment Expression
2.4.1.91 Glycine max the enzyme is expressed not only at the sites of anthocyanin biosynthesis, but also at sites of flavonol glycoside biosynthesis up

General Information

EC Number General Information Comment Organism
2.4.1.91 physiological function the enzyme functions as a 3-O-glucosyltransferase in anthocyanin and flavonol biosynthesis in vivo Glycine max