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Literature summary extracted from

  • Glawischnig, E.; Grün, S.; Frey, M.; Gierl, A.
    Cytochrome P450 monooxygenases of DIBOA biosynthesis: Specificity and conservation among grasses (1999), Phytochemistry, 50, 925-930.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.109 expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3) Zea mays
1.14.14.110 expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3) Zea mays
1.14.14.153 expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3) Zea mays
1.14.14.157 expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3) Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.109 0.031
-
 3-hydroxyindolin-2-one native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.109 0.036
-
 3-hydroxyindolin-2-one recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.110 0.005
-
 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.110 0.007
-
 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.153 0.036
-
 indole native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.153 0.038
-
 indole recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.157 0.01
-
 indolin-2-one recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.157 0.011
-
 benzoxazin-3-one recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.157 0.017
-
 indolin-2-one native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
1.14.14.157 0.018
-
 benzoxazin-3-one native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.109 microsome
-
 Zea mays
-
-
1.14.14.110 microsome
-
 Zea mays
-
-
1.14.14.153 microsome
-
 Zea mays
-
-
1.14.14.157 microsome
-
 Zea mays
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.109 Zea mays
-
 hybrid Blizzard
-
1.14.14.110 Zea mays
-
 hybrid Blizzard
-
1.14.14.153 Zea mays
-
 hybrid Blizzard
-
1.14.14.157 Zea mays
-
 hybrid Blizzard
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.109 0.0002
-
 recombinant enzyme, at pH 7.5 and 25°C Zea mays
1.14.14.110 0.00016
-
 recombinant enzyme, using 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, at pH 7.5 and 25°C Zea mays
1.14.14.153 0.00033
-
 recombinant enzyme, using indole as substrate, at pH 7.5 and 25°C Zea mays
1.14.14.157 0.00194
-
 recombinant enzyme, using indolin-2-one as substrate, at pH 7.5 and 25°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.109 3-hydroxyindolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.110 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.153 indole + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays indolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.157 benzoxazin-3-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays 2-hydroxy-1,4-benzoxazin-3-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.157 indolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays 3-hydroxyindolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.109 BX4
-
 Zea mays
1.14.14.110 BX5
-
 Zea mays
1.14.14.153 BX2
-
 Zea mays
1.14.14.157 BX3
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.109 cytochrome P450
-
 Zea mays
1.14.14.110 cytochrome P450
-
 Zea mays
1.14.14.153 cytochrome P450
-
 Zea mays
1.14.14.157 cytochrome P450
-
 Zea mays
1.14.14.157 NADPH
-
 Zea mays