BRENDA - Enzyme Database show

Attenuation of phosphorylation by deoxycytidine kinase is key to acquired gemcitabine resistance in a pancreatic cancer cell line: targeted proteomic and metabolomic analyses in PK9 cells

Ohmine, K.; Kawaguchi, K.; Ohtsuki, S.; Motoi, F.; Egawa, S.; Unno, M.; Terasaki, T.; Pharm. Res. 29, 2006-2016 (2012)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2',2'-difluorodeoxycytidine
Homo sapiens
i.e. gemcitabine, transport and metabolism in vivo, overview
ADP + 2',2'-difluorodeoxycytidine monophosphate
-
-
?
2.7.1.74
ATP + deoxycytidine
Homo sapiens
-
ADP + dCMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
pancreatic cancer cell
-
Homo sapiens
-
2.7.1.74
PK-9 cell
-
Homo sapiens
-
2.7.1.74
RPK-9 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2',2'-difluorodeoxycytidine
i.e. gemcitabine
723345
Homo sapiens
ADP + 2',2'-difluorodeoxycytidine monophosphate
-
-
-
?
2.7.1.74
ATP + 2',2'-difluorodeoxycytidine
i.e. gemcitabine, transport and metabolism in vivo, overview
723345
Homo sapiens
ADP + 2',2'-difluorodeoxycytidine monophosphate
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723345
Homo sapiens
ADP + dCMP
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.4
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + 2',2'-difluorodeoxycytidine
Homo sapiens
i.e. gemcitabine, transport and metabolism in vivo, overview
ADP + 2',2'-difluorodeoxycytidine monophosphate
-
-
?
2.7.1.74
ATP + deoxycytidine
Homo sapiens
-
ADP + dCMP
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
pancreatic cancer cell
-
Homo sapiens
-
2.7.1.74
PK-9 cell
-
Homo sapiens
-
2.7.1.74
RPK-9 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 2',2'-difluorodeoxycytidine
i.e. gemcitabine
723345
Homo sapiens
ADP + 2',2'-difluorodeoxycytidine monophosphate
-
-
-
?
2.7.1.74
ATP + 2',2'-difluorodeoxycytidine
i.e. gemcitabine, transport and metabolism in vivo, overview
723345
Homo sapiens
ADP + 2',2'-difluorodeoxycytidine monophosphate
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723345
Homo sapiens
ADP + dCMP
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.4
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.7.1.74
malfunction
deoxxycytidine kinase inhibition causes gemcitabine resistance, overview
Homo sapiens
2.7.1.74
metabolism
2',2'-difluorodeoxycytidine, i.e. gemcitabine, is phosphorylated intracellularly via multiple steps, and the triphosphate inhibits DNA synthesis by interfering with incorporation of endogenous dCTP into DNA, deocycytidine kinase catalyzes the first phosphorylation step, overview
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.74
malfunction
deoxxycytidine kinase inhibition causes gemcitabine resistance, overview
Homo sapiens
2.7.1.74
metabolism
2',2'-difluorodeoxycytidine, i.e. gemcitabine, is phosphorylated intracellularly via multiple steps, and the triphosphate inhibits DNA synthesis by interfering with incorporation of endogenous dCTP into DNA, deocycytidine kinase catalyzes the first phosphorylation step, overview
Homo sapiens