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Literature summary extracted from

  • Liang, P.; Zhang, F.; Chen, W.; Hu, X.; Huang, Y.; Li, S.; Ren, M.; He, L.; Li, R.; Li, X.; Xu, J.; Wu, Z.; Lu, G.; Yu, X.
    Identification and biochemical characterization of adenylate kinase 1 from Clonorchis sinensis (2013), Parasitol. Res., 112, 1719-1727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 expressed in Escherichia coli BL21(DE3) cells Clonorchis sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 2.2
-
 ADP in 100 mM Tris–HCl (pH 7.5), 20 mM glucose, 5 mM MgCl2, 10 mM KCl, 2 mM dithiothreitol, at 37°C Clonorchis sinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Ca2+ the enzyme is slightly activated by Ca2+ (85% relative activity at 5 mM) Clonorchis sinensis
2.7.4.3 K+ the enzyme is highly activated by K+, the optimal concentration is 10 mM Clonorchis sinensis
2.7.4.3 Li+ the enzyme is slightly activated by Li+ +(65.87% relative activity at 10 mM) Clonorchis sinensis
2.7.4.3 Mg2+ the enzyme activity is highly dependent on Mg2+, and the optimal concentration of Mg2+ is 2 mM Clonorchis sinensis
2.7.4.3 additional information the enzyme is not activated by Mn2+ Clonorchis sinensis
2.7.4.3 NH4+ the enzyme is highly activated by NH4+ (93.6% relative activity at 5 mM) Clonorchis sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 26700
-
 x * 26700, His12-tagged enzyme ADK1, estimated from SDS-PAGE Clonorchis sinensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Clonorchis sinensis Q8MVS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 His Bind column chromatography Clonorchis sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.3 adult
-
 Clonorchis sinensis
-
2.7.4.3 egg
-
 Clonorchis sinensis
-
2.7.4.3 metacercaria
-
 Clonorchis sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Clonorchis sinensis ATP + AMP
-
 r
2.7.4.3 ATP + AMP
-
 Clonorchis sinensis ADP + ADP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.3 ? x * 26700, His12-tagged enzyme ADK1, estimated from SDS-PAGE Clonorchis sinensis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.3 adenylate kinase 1
-
 Clonorchis sinensis
2.7.4.3 ADK1
-
 Clonorchis sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 37
-
-
 Clonorchis sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 7.5 8
-
 Clonorchis sinensis

Expression

EC Number Organism Comment Expression
2.7.4.3 Clonorchis sinensis the expression level of ADK1 at the stage of excysted metacercaria is higher than those of adult worm (18.8fold), metacercariae (1.5fold) and eggs (5.6fold) up