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Literature summary extracted from

  • Kato, A.; Yasuda, Y.; Kitamura, Y.; Kandeel, M.; Kitade, Y.
    Carbocyclic thymidine derivatives efficiently inhibit Plasmodium falciparum thymidylate kinase (PfTMK) (2012), Parasitol. Int., 61, 501-503.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.9
-
 Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 1-[(1R,2R,3S,4R)-2,3-dihydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
 Plasmodium falciparum
2.7.4.9 1-[(1R,2R,4R,5S)-4-(hydroxymethyl)-6-oxabicyclo[3.1.0]hex-2-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
 Plasmodium falciparum
2.7.4.9 1-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
 Plasmodium falciparum
2.7.4.9 1-[(1R,3S,4R)-3-hydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
 Plasmodium falciparum
2.7.4.9 1-[(1R,4S)-4-(hydroxymethyl)cyclopent-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
 Plasmodium falciparum
2.7.4.9 1-[(1S,3R)-3-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione
-
 Plasmodium falciparum
2.7.4.9 [(1S,4R)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)cyclopent-2-en-1-yl]methyl acetate
-
 Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.9 25000
-
 x * 25000, SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dGMP
-
 Plasmodium falciparum ADP + dGDP
-
 ?
2.7.4.9 ATP + TMP
-
 Plasmodium falciparum ADP + TDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.9 ? x * 25000, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.7.4.9 thymidylate kinase
-
 Plasmodium falciparum
2.7.4.9 TMK
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.007
-
 1-[(1R,4S)-4-(hydroxymethyl)cyclopent-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.02
-
 1-[(1R,4S)-4-(hydroxymethyl)cyclopent-2-en-1-yl]-5-methylpyrimidine-2,4(1H,3H)-dione with TMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.036
-
 [(1S,4R)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)cyclopent-2-en-1-yl]methyl acetate with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.055
-
 1-[(1R,2R,4R,5S)-4-(hydroxymethyl)-6-oxabicyclo[3.1.0]hex-2-yl]-5-methylpyrimidine-2,4(1H,3H)-dione with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.067
-
 1-[(1R,2R,3S,4R)-2,3-dihydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.089
-
 1-[(1S,3R)-3-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.103
-
 1-[(1R,3S,4R)-3-hydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.206
-
 [(1S,4R)-4-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)cyclopent-2-en-1-yl]methyl acetate with TMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.208
-
 1-[(1R,2R,4R,5S)-4-(hydroxymethyl)-6-oxabicyclo[3.1.0]hex-2-yl]-5-methylpyrimidine-2,4(1H,3H)-dione with TMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.246
-
 1-[(1S,3R)-3-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with TMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 0.355
-
 1-[(1R,3S,4R)-3-hydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with TMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 1.54
-
 1-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with dGMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum
2.7.4.9 2.5
-
 1-[(1R,2R,3S,4R)-2,3-dihydroxy-4-(hydroxymethyl)cyclopentyl]-5-methylpyrimidine-2,4(1H,3H)-dione with TMP as substrate, in Tris-HCl (50 mM, pH 7.2), phosphoenolpyruvate (0.5 mM), KCl (40 mM), MgCl2 (2 mM), at 30°C Plasmodium falciparum