BRENDA - Enzyme Database show

Identification and characterization of mitochondrial factors modulating thymidine kinase 2 activity

Sun, R.; Eriksson, S.; Wang, L.; Nucleosides Nucleotides Nucleic Acids 29, 382-385 (2010)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.21
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
2.7.1.21
3'-azido-3'-deoxythymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-deoxy-3'-fluorothymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-fluorothymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.21
expressed in Escherichia coli
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
2.7.1.21
3'-deoxy-3'-fluorothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-fluorothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
2.7.1.21
additional information
at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3‘-azido-3'-deoxythmidine or 3'-fluorothymidine
Homo sapiens
2.7.1.21
additional information
at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3'-azido-3'-deoxythmidine or 3'-fluorothymidine
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0188
-
thymidine
pH and temperature not specified in the publication
Rattus norvegicus
2.7.1.21
0.0414
-
deoxycytidine
pH and temperature not specified in the publication
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.21
mitochondrion
-
Rattus norvegicus
5739
-
2.7.1.21
mitochondrion
-
Homo sapiens
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Homo sapiens
-
-
-
2.7.1.21
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.21
-
Homo sapiens
2.7.1.21
DEAE-Sephadex gel filtration
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.21
liver
-
Homo sapiens
-
2.7.1.21
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + deoxycytidine
-
723302
Homo sapiens
ADP + dCMP
-
-
-
?
2.7.1.21
ATP + deoxycytidine
-
723302
Rattus norvegicus
ADP + dCMP
-
-
-
?
2.7.1.21
ATP + thymidine
-
723302
Homo sapiens
ADP + dTMP
-
-
-
?
2.7.1.21
ATP + thymidine
-
723302
Rattus norvegicus
ADP + dTMP
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.0014
-
3'-deoxy-3'-fluorothymidine
pH and temperature not specified in the publication
Homo sapiens
2.7.1.21
0.0014
-
3'-fluorothymidine
pH and temperature not specified in the publication
Rattus norvegicus
2.7.1.21
0.0039
-
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
pH and temperature not specified in the publication
Homo sapiens
2.7.1.21
0.0039
-
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
pH and temperature not specified in the publication
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.21
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
2.7.1.21
3'-azido-3'-deoxythymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-deoxy-3'-fluorothymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-fluorothymidine
stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
expressed in Escherichia coli
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
2.7.1.21
3'-deoxy-3'-fluorothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Homo sapiens
2.7.1.21
3'-fluorothymidine
inhibits thymidine phosphorylation catalyzed by thymidine kinase 2
Rattus norvegicus
2.7.1.21
additional information
at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3‘-azido-3'-deoxythmidine or 3'-fluorothymidine
Homo sapiens
2.7.1.21
additional information
at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3'-azido-3'-deoxythmidine or 3'-fluorothymidine
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.0014
-
3'-deoxy-3'-fluorothymidine
pH and temperature not specified in the publication
Homo sapiens
2.7.1.21
0.0014
-
3'-fluorothymidine
pH and temperature not specified in the publication
Rattus norvegicus
2.7.1.21
0.0039
-
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
pH and temperature not specified in the publication
Homo sapiens
2.7.1.21
0.0039
-
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine
pH and temperature not specified in the publication
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0188
-
thymidine
pH and temperature not specified in the publication
Rattus norvegicus
2.7.1.21
0.0414
-
deoxycytidine
pH and temperature not specified in the publication
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.21
mitochondrion
-
Rattus norvegicus
5739
-
2.7.1.21
mitochondrion
-
Homo sapiens
5739
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
-
Homo sapiens
2.7.1.21
DEAE-Sephadex gel filtration
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.21
liver
-
Homo sapiens
-
2.7.1.21
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + deoxycytidine
-
723302
Homo sapiens
ADP + dCMP
-
-
-
?
2.7.1.21
ATP + deoxycytidine
-
723302
Rattus norvegicus
ADP + dCMP
-
-
-
?
2.7.1.21
ATP + thymidine
-
723302
Homo sapiens
ADP + dTMP
-
-
-
?
2.7.1.21
ATP + thymidine
-
723302
Rattus norvegicus
ADP + dTMP
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.7.1.21
physiological function
thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells
Homo sapiens
2.7.1.21
physiological function
thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells
Rattus norvegicus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.21
physiological function
thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells
Homo sapiens
2.7.1.21
physiological function
thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells
Rattus norvegicus