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Literature summary extracted from

  • El Yacoubi, B.; Lyons, B.; Cruz, Y.; Reddy, R.; Nordin, B.; Agnelli, F.; Williamson, J.R.; Schimmel, P.; Swairjo, M.A.; Crecy-Lagard, V.
    The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA (2009), Nucleic Acids Res., 37, 2894-2909.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.87 Escherichia coli BL21(DE3) cells transformed with a pET21 derivative (Novagen) vector containing the Escherichia coli yrdC gene Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.87 L-threonine + ATP + bicarbonate Saccharomyces cerevisiae results indicate that the universally conserved SUA5 family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine L-threonylcarbamoyladenylate + diphosphate + H2O threonylcarbamoyladenosine i.e. t6A ?
2.7.7.87 L-threonine + ATP + bicarbonate Escherichia coli results indicate that the universally conserved YrdC family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine L-threonylcarbamoyladenylate + diphosphate + H2O threonylcarbamoyladenosine i.e. t6A ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.87 Escherichia coli P45748
-
-
2.7.7.87 Saccharomyces cerevisiae P32579
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.87 yrdC protein purified by Ni-NTA affinity chromatography, dialyzed against phosphate-buffered saline and concentrated using a YM-3 Centriprep filter Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.87 L-threonine + ATP + bicarbonate results indicate that the universally conserved SUA5 family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine Saccharomyces cerevisiae L-threonylcarbamoyladenylate + diphosphate + H2O threonylcarbamoyladenosine i.e. t6A ?
2.7.7.87 L-threonine + ATP + bicarbonate results indicate that the universally conserved YrdC family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine Escherichia coli L-threonylcarbamoyladenylate + diphosphate + H2O threonylcarbamoyladenosine i.e. t6A ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.87 SUA5
-
 Saccharomyces cerevisiae
2.7.7.87 YrdC
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.7.87 physiological function threonylcarbamoyladenosine is a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro Escherichia coli
2.7.7.87 physiological function threonylcarbamoyladenosine is a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro Saccharomyces cerevisiae