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Literature summary extracted from

  • Crawford, J.M.; Korman, T.P.; Labonte, J.W.; Vagstad, A.L.; Hill, E.A.; Kamari-Bidkorpeh, O.; Tsai, S.C.; Townsend, C.A.
    Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization (2009), Nature, 461, 1139-1143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.221 expression of wild-type and mutant proteins in Escherichia coli strains BL21(DE3) or Rosetta2(DE3) Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.221 7 malonyl-CoA + hexanoyl-[acyl-carrier protein] Aspergillus parasiticus
-
 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.221 Aspergillus parasiticus
-
 gene pksA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.221 recombinant wild-type and mutant proteins from Escherichia coli strains BL21(DE3) or Rosetta2(DE3) Aspergillus parasiticus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.221 7 malonyl-CoA + hexanoyl-[acyl-carrier protein] = 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O reaction mechanism, overview. The SAT domain in PksA selects a hexanoyl starter unit. The MAT domain loads the free ACP with malonyl units. After seven successive condensation events with malonyl-ACP catalysed in te ketoacyl synthase domain, the linear ACP-bound polyketide is cyclized (C4-C9 and C2-C11 cyclization events) and aromatized in the product template domain to give the bicyclic intermediate. The thioesterase domain catalyses C-C cyclization to release anthrone, which undergoes oxidation to the anthraquinone norsolorinic acid, to initiate the complex biosynthetic pathway to aflatoxin B1 Aspergillus parasiticus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.221 7 malonyl-CoA + hexanoyl-[acyl-carrier protein]
-
 Aspergillus parasiticus 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.221 More domain organization of PksA with acyl-carrier protein (ACP), acyl transacylase (AT), dehydrase (DH), enoyl reductase (ER), ketoreductase (KR), ketoacyl synthase (KS), pseudomethyltransferase (PsiMT), starter unit:ACP transacylase (SAT), and thioesterase (TE), structure model, overview Aspergillus parasiticus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.221 PksA
-
 Aspergillus parasiticus

General Information

EC Number General Information Comment Organism
2.3.1.221 metabolism biosynthesis of norsolorinic acid anthrone by PksA, detailed overview Aspergillus parasiticus