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Literature summary extracted from

  • Ohashi, K.; Kawai, S.; Murata, K.
    Identification and characterization of a human mitochondrial NAD kinase (2012), Nat. Commun., 3, 1248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23 expressed in Escherichia coli Rosetta-gami(DE3)pLysS cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 NADH 34% residual activity at 0.5 mM NADH Homo sapiens
2.7.1.23 NADP+ 58% residual activity at 0.5 mM NADP+ Homo sapiens
2.7.1.23 NADPH 17% residual activity at 0.5 mM NADPH Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.22
-
 NAD+ in 100 mM Tris-HCl (pH 8.0), at 37°C Homo sapiens
2.7.1.23 1.7
-
 ATP in 100 mM Tris-HCl (pH 8.0), at 37°C Homo sapiens
2.7.1.23 12
-
 Tetrapolyphosphate in 100 mM Tris-HCl (pH 8.0), at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.23 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 43000
-
 2 * 43000, SDS-PAGE Homo sapiens
2.7.1.23 43300
-
 2 * 43300, calculated from amino acid sequence Homo sapiens
2.7.1.23 75000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23 Talon column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.23 brain
-
 Homo sapiens
-
2.7.1.23 colon
-
 Homo sapiens
-
2.7.1.23 heart highest expression Homo sapiens
-
2.7.1.23 HEK-293A cell
-
 Homo sapiens
-
2.7.1.23 kidney
-
 Homo sapiens
-
2.7.1.23 liver
-
 Homo sapiens
-
2.7.1.23 lung
-
 Homo sapiens
-
2.7.1.23 placenta
-
 Homo sapiens
-
2.7.1.23 skeletal muscle lowest expression Homo sapiens
-
2.7.1.23 small intestine
-
 Homo sapiens
-
2.7.1.23 spleen
-
 Homo sapiens
-
2.7.1.23 stomach
-
 Homo sapiens
-
2.7.1.23 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+ 100% activity Homo sapiens ADP + NADP+
-
 ?
2.7.1.23 ATP + NADH 10% activity compared to NAD+ Homo sapiens ADP + NADPH
-
 ?
2.7.1.23 CTP + NAD+ 56% activity compared to ATP Homo sapiens CDP + NADP+
-
 ?
2.7.1.23 dATP + NAD+ 99% activity compared to ATP Homo sapiens dADP + NADP+
-
 ?
2.7.1.23 dITP + NAD+ 141% activity compared to ATP Homo sapiens dIDP + NADP+
-
 ?
2.7.1.23 GTP + NAD+ 162% activity compared to ATP Homo sapiens GDP + NADP+
-
 ?
2.7.1.23 hexametaphosphate + NAD+ 194% activity compared to ATP Homo sapiens ? + NADP+
-
 ?
2.7.1.23 ITP + NAD+ 104% activity compared to ATP Homo sapiens IDP + NADP+
-
 ?
2.7.1.23 metaphosphate + NAD+ 280% activity compared to ATP Homo sapiens ? + NADP+
-
 ?
2.7.1.23 additional information no activity with NAAD, ADP-ribose, adenosine, 5'-AMP, ADP, sphingosine, diacylglycerol, fructose 6-phosphate, trimetaphosphate, tripolyphosphate, phosphoenolpyruvate, and phosphocreatine Homo sapiens ?
-
 ?
2.7.1.23 tetrapolyphosphate + NAD+ 84% activity compared to ATP Homo sapiens tripolyphosphate + NADP+
-
 ?
2.7.1.23 TTP + NAD+ 87% activity compared to ATP Homo sapiens TDP + NADP+
-
 ?
2.7.1.23 UTP + NAD+ 41% activity compared to ATP Homo sapiens UDP + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 homodimer 2 * 43000, SDS-PAGE Homo sapiens
2.7.1.23 homodimer 2 * 43300, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 C5orf33 protein
-
 Homo sapiens
2.7.1.23 NAD kinase
-
 Homo sapiens
2.7.1.23 NADK
-
 Homo sapiens