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Literature summary extracted from

  • Chelico, L.; Pham, P.; Calabrese, P.; Goodman, M.F.
    APOBEC3G DNA deaminase acts processively 3' -> 5' on single-stranded DNA (2006), Nat. Struct. Mol. Biol., 13, 392-399.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.B9 expressed in baculovirus-infected sf9 insect cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.B9 88000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O Homo sapiens
-
 uracil in single-stranded viral DNA + NH3
-
 ?
3.5.4.B9 additional information Homo sapiens the enzyme binds randomly to single-stranded DNA, then jumps and slides processively to deaminate target motifs. Preferential deamination of the third C is observed in the motif 5'-AAACCCAAA-3' while deamination at the first C is not observed. The replacement of AAA with TTT at the 3' side of CCC results in a 20fold inhibition of deamination. The replacement of AGA by TTT at the 5' side of CCC results in about a 5fold reduction in specific activity. Similar binding constants are observed with single-stranded DNA substrates ranging from 10 to 69 nucleotides whereas binding is reduced sharply for a 9-nucleotide substrate. When confronting partially double-stranded DNA, to which the enzyme cannot bind, sliding is lost but jumping is retained. The enzyme shows catalytic orientational specificity such that deamination occurs predominantly 3'-5' without requiring hydrolysis of a nucleotide cofactor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.B9 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O
-
 Homo sapiens uracil in single-stranded viral DNA + NH3
-
 ?
3.5.4.B9 additional information the enzyme binds randomly to single-stranded DNA, then jumps and slides processively to deaminate target motifs. Preferential deamination of the third C is observed in the motif 5'-AAACCCAAA-3' while deamination at the first C is not observed. The replacement of AAA with TTT at the 3' side of CCC results in a 20fold inhibition of deamination. The replacement of AGA by TTT at the 5' side of CCC results in about a 5fold reduction in specific activity. Similar binding constants are observed with single-stranded DNA substrates ranging from 10 to 69 nucleotides whereas binding is reduced sharply for a 9-nucleotide substrate. When confronting partially double-stranded DNA, to which the enzyme cannot bind, sliding is lost but jumping is retained. The enzyme shows catalytic orientational specificity such that deamination occurs predominantly 3'-5' without requiring hydrolysis of a nucleotide cofactor Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.B9 dimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.B9 APOBEC3G DNA deaminase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.4.B9 physiological function the enzyme is encapsulated by the HIV virion and facilitates restriction of HIV-1 infection in T cells by deaminating cytosines in nascent minus-strand complementary DNA Homo sapiens