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Literature summary extracted from

  • Woodward, R.; Yi, W.; Li, L.; Zhao, G.; Eguchi, H.; Sridhar, P.R.; Guo, H.; Song, J.K.; Motari, E.; Cai, L.; Kelleher, P.; Liu, X.; Han, W.; Zhang, W.; Ding, Y.; Li, M.; Wang, P.G.
    In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz (2010), Nat. Chem. Biol., 6, 418-423.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.122 inner membrane
-
 Escherichia coli
-
-
2.4.1.306 inner membrane
-
 Escherichia coli
-
-
2.4.1.308 inner membrane
-
 Escherichia coli
-
-
2.4.1.309 inner membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.122 UDP-alpha-D-galactose + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol Escherichia coli biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 UDP + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.122 UDP-alpha-D-galactose + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol Escherichia coli O86:B7 biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 UDP + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli O86:B7 biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol Escherichia coli biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol Escherichia coli O86:B7 biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol Escherichia coli biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol Escherichia coli O86:B7 biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.122 Escherichia coli Q4KXC9
-
-
2.4.1.122 Escherichia coli O86:B7 Q4KXC9
-
-
2.4.1.306 Escherichia coli Q5J7D6
-
-
2.4.1.306 Escherichia coli O86:B7 Q5J7D6
-
-
2.4.1.308 Escherichia coli Q58YV9
-
-
2.4.1.308 Escherichia coli O86:B7 Q58YV9
-
-
2.4.1.309 Escherichia coli Q5JBG6
-
-
2.4.1.309 Escherichia coli O86:B7 Q5JBG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.122
-
 Escherichia coli
2.4.1.306
-
 Escherichia coli
2.4.1.308
-
 Escherichia coli
2.4.1.309
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.122 UDP-alpha-D-galactose + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli UDP + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.122 UDP-alpha-D-galactose + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli O86:B7 UDP + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI Escherichia coli UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI Escherichia coli O86:B7 UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli O86:B7 GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli O86:B7 UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.122 WbnJ
-
 Escherichia coli
2.4.1.306 WbnH
-
 Escherichia coli
2.4.1.308 WbnK
-
 Escherichia coli
2.4.1.309 WbnI
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.122 22
-
 assay at Escherichia coli
2.4.1.306 22
-
 assay at Escherichia coli
2.4.1.308 22
-
 assay at Escherichia coli
2.4.1.309 22
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.122 7
-
 assay at Escherichia coli
2.4.1.306 7
-
 assay at Escherichia coli
2.4.1.308 7
-
 assay at Escherichia coli
2.4.1.309 7
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.307 physiological function the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli
2.4.1.308 physiological function the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli
2.4.1.309 physiological function the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86 Escherichia coli