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Literature summary extracted from

  • Park, S.H.; Park, K.H.; Oh, B.C.; Alli, I.; Lee, B.H.
    Expression and characterization of an extremely thermostable beta-glycosidase (mannosidase) from the hyperthermophilic archaeon Pyrococcus furiosus DSM3638 (2011), N. Biotechnol., 28, 639-648.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B28 food industry the hyperthermostable beta-glycosidase may be useful for food and pharmaceutical applications Pyrococcus furiosus
3.2.1.B28 pharmacology the hyperthermostable beta-glycosidase may be useful for food and pharmaceutical applications Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B28 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B28 0.04
-
 lactose pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.07
-
 cellobiose pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.09
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.09
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.12
-
 2-nitrophenyl beta-D-galactopyranoside pH 5.0, 100°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B28 56326
-
 4 * 56326, calculated from sequence Pyrococcus furiosus
3.2.1.B28 57000
-
 4 * 57000, SDS-PAGE Pyrococcus furiosus
3.2.1.B28 230000
-
 non-denaturing PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus Q8U3U9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B28
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-galactopyranoside + H2O 37% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside Pyrococcus furiosus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O 114% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-xylopyranoside + H2O 37% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside Pyrococcus furiosus 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.B28 cellobiose + H2O
-
 Pyrococcus furiosus 2 beta-D-glucose
-
 ?
3.2.1.B28 lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 ?
3.2.1.B28 mannooligosaccharide + H2O hydrolysis of mannose from the reducing end Pyrococcus furiosus ?
-
 ?
3.2.1.B28 additional information the enzyme shows transglycosylation and transgalactosylation activities toward cellobiose, lactose and mannooligosaccharides that could produce galactooligosaccharides and maltooligosaccharides. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B28 tetramer 4 * 57000, SDS-PAGE Pyrococcus furiosus
3.2.1.B28 tetramer 4 * 56326, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B28 PFTG
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 100
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B28 70 110 70°C: about 50% of maximal activity, 110°C: about 45% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B28 85
-
 2 h, stable Pyrococcus furiosus
3.2.1.B28 90
-
 half-life: 398 min Pyrococcus furiosus
3.2.1.B28 95
-
 half-life: 114 min Pyrococcus furiosus
3.2.1.B28 100
-
 1 h, 73% loss of activity Pyrococcus furiosus
3.2.1.B28 110
-
 thermal transition midpoint Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B28 0.08
-
 lactose pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.16
-
 cellobiose pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.23
-
 2-nitrophenyl beta-D-galactopyranoside pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.26
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 0.3
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 100°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 5
-
 sodium citrate buffer Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B28 4.5 6 pH 4.5: about 85% of maximal activity, pH 6.0: about 60% of maximal activity Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B28 1.82
-
 lactose pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 1.89
-
 2-nitrophenyl beta-D-galactopyranoside pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 2.28
-
 cellobiose pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 2.9
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 100°C Pyrococcus furiosus
3.2.1.B28 3.02
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 100°C Pyrococcus furiosus