Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, Y.; Yu, H.; Chen, Y.; Lau, K.; Cai, L.; Cao, H.; Tiwari, V.; Qu, J.; Thon, V.; Wang, P.; Chen, X.
    Substrate promiscuity of N-acetylhexosamine 1-kinases (2011), Molecules, 16, 6396-6407.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.162 expression in Escherichia coli Bifidobacterium longum subsp. infantis
2.7.1.162 expression in Escherichia coli Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.162 0.06
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 0.06
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 0.06
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 0.08
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 0.08
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 0.09
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 0.1
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 0.11
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.162 Mg2+ optimal concentration 1mM Bifidobacterium longum subsp. infantis
2.7.1.162 Mg2+ optimal concentration 1mM Bifidobacterium longum
2.7.1.162 additional information divalent cation required for activity Bifidobacterium longum subsp. infantis
2.7.1.162 additional information divalent cation required for activity Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.162 Bifidobacterium longum
-
-
-
2.7.1.162 Bifidobacterium longum ATCC 55813
-
-
-
2.7.1.162 Bifidobacterium longum subsp. infantis
-
-
-
2.7.1.162 Bifidobacterium longum subsp. infantis ATCC 15697
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.162 ATP + 2-deoxy-2-azido-D-mannose 0.75 microM, 40.2% conversion Bifidobacterium longum ADP + 2-deoxy-2-azido-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 2-deoxy-2-azido-D-mannose 0.75 microM, 53.3% conversion Bifidobacterium longum subsp. infantis ADP + 2-deoxy-2-azido-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 2-deoxy-2-azido-D-mannose 0.75 microM, 53.3% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + 2-deoxy-2-azido-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 2-deoxy-2-fluoro-D-mannose 0.75 microM, 44.4% conversion Bifidobacterium longum subsp. infantis ADP + 2-deoxy-2-fluoro-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 2-deoxy-2-fluoro-D-mannose 0.75 microM, 47.0% conversion Bifidobacterium longum ADP + 2-deoxy-2-fluoro-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 2-deoxy-D-glucose 0.75 microM, 28.4% conversion Bifidobacterium longum ADP + 2-deoxy-alpha-D-glucose 1-phosphate
-
 ?
2.7.1.162 ATP + 2-deoxy-D-glucose 0.75 microM, 44.8% conversion Bifidobacterium longum subsp. infantis ADP + 2-deoxy-alpha-D-glucose 1-phosphate
-
 ?
2.7.1.162 ATP + 4-deoxy-D-mannose 0.75 microM, 23.9% conversion Bifidobacterium longum ADP + 4-deoxy-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 4-deoxy-D-mannose 0.75 microM, 37.1% conversion Bifidobacterium longum subsp. infantis ADP + 4-deoxy-alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 23.4% conversion Bifidobacterium longum ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 37.2% conversion Bifidobacterium longum subsp. infantis ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 37.2% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-deoxy-6-azido-N-acetyl-D-glucosamine 0.75 microM, 23.4% conversion Bifidobacterium longum ATCC 55813 ADP + 6-deoxy-6-azido-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-deoxy-6-methyl-N-acetyl-D-glucosamine 0.75 microM, 36.3% conversion Bifidobacterium longum ADP + 6-deoxy-6-methyl-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-deoxy-6-methyl-N-acetyl-D-glucosamine 0.75 microM, 41.8% conversion Bifidobacterium longum subsp. infantis ADP + 6-deoxy-6-methyl-N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-methyl-N-acetyl-D-mannosamine 0.75 microM, 28.6% conversion Bifidobacterium longum ADP + 6-methyl-N-acetyl-alpha-D-mannosamine 1-phosphate
-
 ?
2.7.1.162 ATP + 6-methyl-N-acetyl-D-mannosamine 0.75 microM, 32.6% conversion Bifidobacterium longum subsp. infantis ADP + 6-methyl-N-acetyl-alpha-D-mannosamine 1-phosphate
-
 ?
2.7.1.162 ATP + D-mannose 0.75 microM, 37.1% conversion Bifidobacterium longum ADP + alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + D-mannose 0.75 microM, 68.0% conversion Bifidobacterium longum subsp. infantis ADP + alpha-D-mannose 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum subsp. infantis ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum subsp. infantis ATCC 15697 ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-galactosamine
-
 Bifidobacterium longum ATCC 55813 ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 35.4% conversion Bifidobacterium longum subsp. infantis ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 42.3% conversion Bifidobacterium longum ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 35.4% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-acetyl-alpha-D-glucosamine 0.75 microM, 42.3% conversion Bifidobacterium longum ATCC 55813 ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-benzoyl-alpha-D-galactosamine 0.75 microM, 51.9% conversion Bifidobacterium longum ADP + N-benzoyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-benzoyl-alpha-D-galactosamine 0.75 microM, 62.2% conversion Bifidobacterium longum subsp. infantis ADP + N-benzoyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-butanoyl-D-galactosamine 0.75 microM, 24.0% conversion Bifidobacterium longum ADP + N-butanoyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-butanoyl-D-galactosamine 0.75 microM, 24.0% conversion Bifidobacterium longum ATCC 55813 ADP + N-butanoyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-butanoyl-D-glucosamine 0.75 microM, 20.9% conversion Bifidobacterium longum subsp. infantis ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-butanoyl-D-glucosamine 0.75 microM, 35.0% conversion Bifidobacterium longum ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-butanoyl-D-glucosamine 0.75 microM, 20.9% conversion Bifidobacterium longum subsp. infantis ATCC 15697 ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-butanoyl-D-glucosamine 0.75 microM, 35.0% conversion Bifidobacterium longum ATCC 55813 ADP + N-butanoyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.162 ATP + N-trifluoroacetyl-alpha-D-galactosamine 0.75 microM, 21.8% conversion Bifidobacterium longum ADP + N-trifluoroacetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.162 NahK
-
 Bifidobacterium longum subsp. infantis
2.7.1.162 NahK
-
 Bifidobacterium longum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.162 0.38
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 0.46
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 0.48
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 0.57
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 0.95
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 1.1
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 1.1
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 1.3
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.162 7 8
-
 Bifidobacterium longum subsp. infantis
2.7.1.162 7 8
-
 Bifidobacterium longum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.162 4.8
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 5.1
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 7.1
-
 N-acetyl-alpha-D-galactosamine pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 8
-
 ATP cosubstrate N-acetyl-alpha-D-galactosamine, pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 11
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 11.8
-
 ATP cosubstrate N-acetyl-alpha-D-glucosamine, pH 8.0, 37°C Bifidobacterium longum
2.7.1.162 15.8
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum subsp. infantis
2.7.1.162 18.3
-
 N-acetyl-alpha-D-glucosamine pH 8.0, 37°C Bifidobacterium longum