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Literature summary extracted from

  • Wang, Q.; Ding, P.; Perepelov, A.V.; Xu, Y.; Wang, Y.; Knirel, Y.A.; Wang, L.; Feng, L.
    Characterization of the dTDP-D-fucofuranose biosynthetic pathway in Escherichia coli O52 (2008), Mol. Microbiol., 70, 1358-1367.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B28 overexpression in Escherichia coli BL21 Escherichia coli
5.4.99.59 overexpression in Escherichia coli BL21 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B28 Co2+ 5 mM, 11% inhibition Escherichia coli
1.1.1.B28 Cu2+ 5 mM, 88% inhibition Escherichia coli
5.4.99.59 Cu2+ 5 mM, complete inhibition Escherichia coli
5.4.99.59 Mn2+ 5 mM, 43% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B28 0.38
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose 37°C, pH 7.4 Escherichia coli
1.1.1.B28 0.84
-
 NADH 37°C, pH 7.4 Escherichia coli
1.1.1.B28 0.89
-
 NADPH 37°C, pH 7.4 Escherichia coli
5.4.99.59 3.43
-
 dTDP-alpha-D-fucopyranose pH 7.4, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B28 additional information Fcf1 is a divalent cation-independent enzyme Escherichia coli
5.4.99.59 additional information Fcf2 is an divalent cation-independent enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B28 40700
-
 x * 40700, His6-tagged fusion protein, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADP+ Escherichia coli the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
 ?
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADP+ Escherichia coli O52 the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
 ?
5.4.99.59 dTDP-alpha-D-fucopyranose Escherichia coli the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen dTDP-beta-D-fuco-(1->4)-furanose
-
 ?
5.4.99.59 dTDP-alpha-D-fucopyranose Escherichia coli O52 the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen dTDP-beta-D-fuco-(1->4)-furanose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B28 Escherichia coli
-
-
-
1.1.1.B28 Escherichia coli O52
-
-
-
5.4.99.59 Escherichia coli Q6E7F1
-
-
5.4.99.59 Escherichia coli O52 Q6E7F1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B28 His6-tagged fusion protein Escherichia coli
5.4.99.59 His6-tagged fusion protein Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.B28 -20°C, in 50% glycerol, stable for at least 5 months Escherichia coli
5.4.99.59 -20°C, in 50% glycerol, stable for at least 2 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADH + H+ NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH Escherichia coli dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
-
 ?
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADH + H+ NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH Escherichia coli O52 dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
-
 ?
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADP+ the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway Escherichia coli dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
 ?
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADP+ the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway Escherichia coli O52 dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
 ?
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADPH + H+ NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH Escherichia coli dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
-
 ?
1.1.1.B28 dTDP-alpha-D-fucopyranose + NADPH + H+ NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH Escherichia coli O52 dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
-
 ?
5.4.99.59 dTDP-alpha-D-fucopyranose
-
 Escherichia coli dTDP-beta-D-fuco-(1->4)-furanose
-
 ?
5.4.99.59 dTDP-alpha-D-fucopyranose the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen Escherichia coli dTDP-beta-D-fuco-(1->4)-furanose
-
 ?
5.4.99.59 dTDP-alpha-D-fucopyranose
-
 Escherichia coli O52 dTDP-beta-D-fuco-(1->4)-furanose
-
 ?
5.4.99.59 dTDP-alpha-D-fucopyranose the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen Escherichia coli O52 dTDP-beta-D-fuco-(1->4)-furanose
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B28 ? x * 40700, His6-tagged fusion protein, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B28 dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase
-
 Escherichia coli
1.1.1.B28 Fcf1
-
 Escherichia coli
5.4.99.59 Fcf2
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B28 15 37
-
 Escherichia coli
5.4.99.59 15 37
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.B28 4 50 4°C: 47% of maximal activity, 50°C: 36% of maximal activity Escherichia coli
5.4.99.59 4 50 4°C: 17% of maximal activity, 50°C: 49% of maximal activity Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.B28 2.78
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose 37°C, pH 7.4 Escherichia coli
1.1.1.B28 6.76
-
 NADPH 37°C, pH 7.4 Escherichia coli
1.1.1.B28 26.67
-
 NADH 37°C, pH 7.4 Escherichia coli
5.4.99.59 0.056
-
 dTDP-alpha-D-fucopyranose pH 7.4, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B28 7.4
-
 assay at Escherichia coli
5.4.99.59 7.4
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B28 NADH NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH Escherichia coli
1.1.1.B28 NADPH NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH Escherichia coli

General Information

EC Number General Information Comment Organism
1.1.1.B28 malfunction mutant strains H1862 lacking fcf1. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf1 is required for the synthesis of the O-antigen in Escherichia coli O52 Escherichia coli
1.1.1.B28 physiological function the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O-antigen Escherichia coli
5.4.99.59 malfunction mutant strains H1863 lacking fcf2. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf2 is required for the synthesis of the O antigen in Escherichia coli O52 Escherichia coli
5.4.99.59 physiological function the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.B28 7.4
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose 37°C, pH 7.4 Escherichia coli
1.1.1.B28 7.6
-
 NADPH 37°C, pH 7.4 Escherichia coli
1.1.1.B28 31.75
-
 NADH 37°C, pH 7.4 Escherichia coli
5.4.99.59 0.016
-
 dTDP-alpha-D-fucopyranose pH 7.4, 37°C Escherichia coli