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Literature summary extracted from

  • Shibayama, K.; Wachino, J.; Arakawa, Y.; Saidijam, M.; Rutherford, N.; Henderson, P.
    Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: Possible significance in the pathophysiology of the organism (2007), Mol. Microbiol., 64, 396-406.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.13 expression in Escherichia coli Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.13 6-diazo-5-oxo-L-norleucine pretreatment of cells completely abolishes the extracellular hydrolysis of lgutathione and glutamine Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.13 0.00133
-
 L-glutamine pH 6.0, 37°C Helicobacter pylori
3.4.19.13 0.181
-
 L-glutamine pH 8.0, 37°C Helicobacter pylori
3.4.19.13 0.23
-
 L-glutamine pH 7.0, 37°C Helicobacter pylori
3.4.19.13 0.52
-
 L-glutamine pH 9.0, 37°C Helicobacter pylori
3.4.19.13 0.918
-
 glutathione pH 6.0, 37°C Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.19.13 periplasm
-
 Helicobacter pylori
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.13 Helicobacter pylori O25743 enzyme catalyses both gamma-glutamyl transfer, reaction of EC 2.3.2.2, and hydrolysis reactions at the same time
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.13 recombinant protein Helicobacter pylori

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.19.13 glutathione + H2O = L-cysteinylglycine + L-glutamate ping-pong kinetics mechanism, in which an acyl enzyme intermediate is formed Helicobacter pylori
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.13 glutathione + H2O
-
 Helicobacter pylori L-cysteinylglycine + L-glutamate
-
 ?
3.4.19.13 L-glutamine + H2O
-
 Helicobacter pylori L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.19.13 hp1118
-
 Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.13 7 9.3 hydrolysis activity is optimal and almost constant within Helicobacter pylori

General Information

EC Number General Information Comment Organism
3.4.19.13 physiological function one of the principle physiological functions of the enzyme is to enable Helicobacter pylori cells to utilize extracellular glutamine and glutathione as a source of glutamate. Helicobacter pylori cells are unable to take up extracellular glutamine and glutathione directly. Instead, these substances are hydrolysed to glutamate by the action of the enzyme outside the cells Helicobacter pylori