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Literature summary extracted from

  • Matsuura, A.; Yoon, J.Y.; Yoon, H.J.; Lee, H.H.; Suh, S.W.
    Crystal structure of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii (2012), Mol. Cells, 34, 407-412.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 expressed in Escherichia coli Rosetta2 (DE3) pLysS Pyrococcus horikoshii
4.3.3.6 overexpressed in Escherichia coli strain Rosetta2 (DE3) Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.6 2-methyl-2,4-pentanediol Pyrococcus horikoshii
4.3.3.6 crystallized at 23°C using 2-methyl-2,4-pentanediol as a precipitant. Crystals of apo and ribose 5'-phosphate complex forms of PdxS diffract to 2.7 A and 3.1 A resolution, respectively, and belong to the monoclinic space group P2(1) Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Pyrococcus horikoshii O59080
-
-
4.3.3.6 Pyrococcus horikoshii OT-3 O59080
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
-
 Pyrococcus horikoshii pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
-
 Pyrococcus horikoshii OT-3 pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.6 hexamer PdxS exists as a hexamer or dodecamer depending on species and makes a 1:1 complex with PdxT. Pyrococcus horikoshii PdxS forms hexamer in solution. It has a 37 amino acids insertion region, which is found in some archaeal PdxS proteins. This additional insertion perturbs dodecamer formation of Pyrococcus horikoshii PdxS Pyrococcus horikoshii
4.3.3.6 homohexamer gel filtration Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 PdxS
-
 Pyrococcus horikoshii
4.3.3.6 pyridoxal biosynthesis lyase PdxS
-
 Pyrococcus horikoshii