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Literature summary extracted from

  • Manandhar, S.; Ricarte, F.; Cocca, S.; Gharakhanian, E.
    Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole (2013), Mol. Cell. Biol., 33, 526-542.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 gene ENV7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His6-tagged Env7 in Escherichia coli strain BL21(DE3)(pLysS), functional expression of HA- and GFP-tagged Env7 and overexpression of wild-type untagged Env7 in yeast cells. HA- and GFP-tagged Env7 localize to vacuolar membranes, and Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 C13S/C14S/C15S site-directed mutagenesis Saccharomyces cerevisiae
2.7.11.1 E269A site-directed mutagenesis, the mutant is degraded in a proteasome-dependent manner, its kinase function is abolished when stabilized Saccharomyces cerevisiae
2.7.11.1 E269D site-directed mutagenesis Saccharomyces cerevisiae
2.7.11.1 E269S site-directed mutagenesis Saccharomyces cerevisiae
2.7.11.1 K69R/K70M site-directed mutagenesis Saccharomyces cerevisiae
2.7.11.1 K69R/K70R site-directed mutagenesis Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 endosome at late endosome and vacuole interface Saccharomyces cerevisiae 5768
-
2.7.11.1 Golgi apparatus excessive overexpression of GFP-tagged Env7 leads to additional localization to the Golgi apparatus Saccharomyces cerevisiae 5794
-
2.7.11.1 membrane the enzyme's N-terminal Cys13 to -15 track is essential for Env7 vacuolar membrane anchoring and kinase activity Saccharomyces cerevisiae 16020
-
2.7.11.1 vacuole at late endosome and vacuole interface, GFP- and HA-tagged recombinant Env7s are vacuolar membrane proteins Saccharomyces cerevisiae 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ dependent on Saccharomyces cerevisiae
2.7.11.1 Mn2+ can substitute for Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 42000
-
 x * 42000, about, His6-tagged enzyme, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Saccharomyces cerevisiae
-
 gene ENV7
-
2.7.11.1 Saccharomyces cerevisiae BY4742
-
 gene ENV7
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 lipoprotein Env7 is a palmitoylated protein kinase Saccharomyces cerevisiae
2.7.11.1 phosphoprotein the enzyme performs autophosphorylation, also recombinant HA- and His-tagged Env7 proteins, and immunopreciptated HA-tagged Env7, are autophosphorylated in vitro Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 recombinant His6-tagged Env7 from Escherichia coli strain BL21(DE3)(pLysS) by nickel affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + beta-casein
-
 Saccharomyces cerevisiae ADP + phosphorylated beta-casein
-
 ?
2.7.11.1 ATP + beta-casein
-
 Saccharomyces cerevisiae BY4742 ADP + phosphorylated beta-casein
-
 ?
2.7.11.1 ATP + histone H1
-
 Saccharomyces cerevisiae ADP + phosphorylated histone H1
-
 ?
2.7.11.1 ATP + histone H1
-
 Saccharomyces cerevisiae BY4742 ADP + phosphorylated histone H1
-
 ?
2.7.11.1 ATP + histone H2B
-
 Saccharomyces cerevisiae ADP + phosphorylated histone H2B
-
 ?
2.7.11.1 ATP + histone H2B
-
 Saccharomyces cerevisiae BY4742 ADP + phosphorylated histone H2B
-
 ?
2.7.11.1 ATP + histone H3
-
 Saccharomyces cerevisiae ADP + phosphorylated histone H3
-
 ?
2.7.11.1 ATP + histone H3
-
 Saccharomyces cerevisiae BY4742 ADP + phosphorylated histone H3
-
 ?
2.7.11.1 ATP + myelin basic protein
-
 Saccharomyces cerevisiae ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.1 ATP + myelin basic protein
-
 Saccharomyces cerevisiae BY4742 ADP + phosphorylated myelin basic protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 ? x * 42000, about, His6-tagged enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 Env7
-
 Saccharomyces cerevisiae
2.7.11.1 serine/threonine kinase
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.7.11.1 evolution gene ENV7 is a widely conserved orthologue of the serine/threonine kinase 16 (STK16) gene, phylogenetic anaysis Saccharomyces cerevisiae
2.7.11.1 malfunction ENV7 deletion leads to internal accumulation of precursor carboxypeptidase Y, a vacuolar hydrolytic enzyme that transits the rough endoplasmic reticulum, Golgi apparatus, and late endosome prior to arrival at the vacuole, where it is processed to mature form via PEP4-dependent proteolytic cleavage. env7DELTA is defective in maintaining fragmented vacuoles during hyperosmotic response and in buds Saccharomyces cerevisiae
2.7.11.1 additional information the enzyme's N-terminal Cys13 to -15 track is essential for Env7 vacuolar membrane anchoring and kinase activity Saccharomyces cerevisiae
2.7.11.1 physiological function possible role for STK16-related kinases in regulation of the endomembrane architecture. Env7 negatively regulates fusion at the vacuole membrane Saccharomyces cerevisiae