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Literature summary extracted from

  • Breda, A.; Rosado, L.; Lorenzini, D.; Basso, L.; Santos, D.
    Molecular, kinetic and thermodynamic characterization of Mycobacterium tuberculosis orotate phosphoribosyltransferase (2012), Mol. Biosyst., 8, 572-586.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.10 expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 diphosphate product inhibition Mycobacterium tuberculosis
2.4.2.10 orotidine 5'-phosphate product inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 0.0026
-
orotidine 5'-phosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.009
-
5-phospho-alpha-D-ribose 1-diphosphate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.0094
-
Orotate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.01
-
Orotate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.016
-
diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.096
-
5-phospho-alpha-D-ribose 1-diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.10 Mg2+
-
Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 18761
-
2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration Mycobacterium tuberculosis
2.4.2.10 20000
-
2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration Mycobacterium tuberculosis
2.4.2.10 38000
-
gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Mycobacterium tuberculosis P9WHK9
-
-
2.4.2.10 Mycobacterium tuberculosis H37Rv P9WHK9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10 anion exchange chromatography, gel filtration, hydrophobic interaction chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 105
-
pH 8.0, 25°C Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
2.4.2.10 -80°C, stable up to 12 months, 5% glycerol, pH 8.0 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Mycobacterium tuberculosis orotidine 5'-phosphate + diphosphate
-
r
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Mycobacterium tuberculosis H37Rv orotidine 5'-phosphate + diphosphate
-
r
2.4.2.10 orotidine 5'-phosphate + diphosphate
-
Mycobacterium tuberculosis orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.10 orotidine 5'-phosphate + diphosphate
-
Mycobacterium tuberculosis H37Rv orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.2.10 homodimer 2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.10 orotate phosphoribosyltransferase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.10 0.28
-
diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.28
-
orotidine 5'-phosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.6
-
Orotate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.61
-
Orotate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.10 0.00044
-
orotidine 5'-phosphate mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.001
-
orotidine 5'-phosphate mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.0074
-
orotidine 5'-phosphate mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.026
-
diphosphate mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.029
-
diphosphate mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.035
-
orotidine 5'-phosphate noncompetitive, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.108
-
diphosphate noncompetitive, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 0.209
-
diphosphate mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.10 6
-
5-phospho-alpha-D-ribose 1-diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 6.6
-
5-phospho-alpha-D-ribose 1-diphosphate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 10
-
diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 60
-
Orotate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 64
-
Orotate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
2.4.2.10 100
-
orotidine 5'-phosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis