EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.10 | expressed in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.10 | diphosphate | product inhibition | Mycobacterium tuberculosis | |
2.4.2.10 | orotidine 5'-phosphate | product inhibition | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.10 | 0.0026 | - |
orotidine 5'-phosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.009 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.0094 | - |
Orotate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.01 | - |
Orotate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.016 | - |
diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.096 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.10 | Mg2+ | - |
Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.10 | 18761 | - |
2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration | Mycobacterium tuberculosis |
2.4.2.10 | 20000 | - |
2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration | Mycobacterium tuberculosis |
2.4.2.10 | 38000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.10 | Mycobacterium tuberculosis | P9WHK9 | - |
- |
2.4.2.10 | Mycobacterium tuberculosis H37Rv | P9WHK9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.10 | anion exchange chromatography, gel filtration, hydrophobic interaction chromatography | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.10 | 105 | - |
pH 8.0, 25°C | Mycobacterium tuberculosis |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.10 | -80°C, stable up to 12 months, 5% glycerol, pH 8.0 | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.10 | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | orotidine 5'-phosphate + diphosphate | - |
r | |
2.4.2.10 | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | orotidine 5'-phosphate + diphosphate | - |
r | |
2.4.2.10 | orotidine 5'-phosphate + diphosphate | - |
Mycobacterium tuberculosis | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.10 | orotidine 5'-phosphate + diphosphate | - |
Mycobacterium tuberculosis H37Rv | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.10 | homodimer | 2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.10 | orotate phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.10 | 0.28 | - |
diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.28 | - |
orotidine 5'-phosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.6 | - |
Orotate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.61 | - |
Orotate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.10 | 0.00044 | - |
orotidine 5'-phosphate | mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.001 | - |
orotidine 5'-phosphate | mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.0074 | - |
orotidine 5'-phosphate | mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.026 | - |
diphosphate | mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.029 | - |
diphosphate | mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.035 | - |
orotidine 5'-phosphate | noncompetitive, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.108 | - |
diphosphate | noncompetitive, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 0.209 | - |
diphosphate | mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.10 | 6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 6.6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 10 | - |
diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 60 | - |
Orotate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 64 | - |
Orotate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
2.4.2.10 | 100 | - |
orotidine 5'-phosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis |