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Literature summary extracted from

  • Jin, M.M.; Wang, P.; Li, X.; Zhao, X.Y.; Xu, L.; Song, P.; Zhu, G.P.
    Biochemical characterization of NADP+-dependent isocitrate dehydrogenase from Microcystis aeruginosa PCC7806 (2013), Mol. Biol. Rep., 40, 2995-3002.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 gene icd, sequence comparisons, expression of His6-tagged in Escherichia coli strain Rosetta (DE3) Microcystis aeruginosa
1.1.1.42 gene icd, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) Microcystis aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 Ca2+
-
 Microcystis aeruginosa
1.1.1.42 Co2+
-
 Microcystis aeruginosa
1.1.1.42 Cu2+ complete inhibition Microcystis aeruginosa
1.1.1.42 K+ slight inhibition Microcystis aeruginosa
1.1.1.42 Li+ slight inhibition Microcystis aeruginosa
1.1.1.42 Na+ slight inhibition Microcystis aeruginosa
1.1.1.42 Ni2+
-
 Microcystis aeruginosa
1.1.1.42 Rb+ slight inhibition Microcystis aeruginosa
1.1.1.42 Zn2+ strong inhibition Microcystis aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 additional information
-
 additional information kinetic analysis Microcystis aeruginosa
1.1.1.42 0.0322
-
 NADP+ with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 0.0324
-
 NADP+ with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.0326
-
 DL-isocitrate with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.0326
-
 DL-isocitrate with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 0.0717
-
 NADP+ with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.0797
-
 NADP+ with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 0.1243
-
 DL-isocitrate with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.1243
-
 DL-isocitrate with Mn2+, pH 7.5, 37°C Microcystis aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Ca2+ activates poorly, 2.48% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Co2+ activates, 10.22% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 K+ activates slightly, 10.18% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Li+ activates slightly, 12.63% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Mg2+ can partially substitute for Mn2+ Microcystis aeruginosa
1.1.1.42 Mg2+ activates, 72.45% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Mn2+ required Microcystis aeruginosa
1.1.1.42 Mn2+ activates, required, best divalent cation Microcystis aeruginosa
1.1.1.42 additional information the enzyme is completely divalent cation dependent. As compared with Mn2+, MaIDH shows about 2.5times and 4times higher affinities (1/Km) to NADP+ and DL-isocitrate with Mg2+, Cu2+ does not activate the enzyme at all Microcystis aeruginosa
1.1.1.42 Na+ activates slightly, 10.70% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Ni2+ activates poorly, 1.40% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Rb+ activates slightly, 20.67% activity compared to Mn2+ Microcystis aeruginosa
1.1.1.42 Zn2+ activates poorly, 4.23% activity compared to Mn2+ Microcystis aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 52600
-
-
 Microcystis aeruginosa
1.1.1.42 52600
-
 2 * 52600 Microcystis aeruginosa
1.1.1.42 105000
-
 gel filtration Microcystis aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 DL-isocitrate + NADP+ Microcystis aeruginosa
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 DL-isocitrate + NADP+ Microcystis aeruginosa
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Microcystis aeruginosa
-
-
-
1.1.1.42 Microcystis aeruginosa A8YGS2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography Microcystis aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 DL-isocitrate + NADP+
-
 Microcystis aeruginosa 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 DL-isocitrate + NADP+
-
 Microcystis aeruginosa 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.42 homodimer 2 * 52600 Microcystis aeruginosa
1.1.1.42 homodimer 2 * 52600, SDS-PAGE Microcystis aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 ICD
-
 Microcystis aeruginosa
1.1.1.42 NADP+-dependent IDH
-
 Microcystis aeruginosa
1.1.1.42 NADP+-dependent isocitrate dehydrogenase
-
 Microcystis aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 45
-
 with Mg2+ Microcystis aeruginosa
1.1.1.42 45
-
 with Mn2+ Microcystis aeruginosa
1.1.1.42 50
-
 with Mg2+ Microcystis aeruginosa
1.1.1.42 50
-
 with Mn2+ Microcystis aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.42 25 60 activity range Microcystis aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 30
-
 purified recombinant enzyme, 20 min, stable Microcystis aeruginosa
1.1.1.42 40
-
 purified recombinant enzyme, 20 min, loss of 50% of enzyme activity, with Mg2+ Microcystis aeruginosa
1.1.1.42 45
-
 purified recombinant enzyme, 20 min, loss of 50% of enzyme activity, with Mn2+ Microcystis aeruginosa
1.1.1.42 45
-
 purified enzyme, 20 min, 50% loss of enzyme activity Microcystis aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 10.29
-
 DL-isocitrate with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 10.92
-
 DL-isocitrate with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 25.36
-
 NADP+ with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 25.36
-
 NADP+ with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 43.21
-
 DL-isocitrate with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 43.21
-
 DL-isocitrate with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 48.88
-
 NADP+ with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 48.88
-
 NADP+ with Mn2+, pH 7.5, 37°C Microcystis aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.5
-
 with Mn2+ or Mg2+ Microcystis aeruginosa
1.1.1.42 7.5
-
 the recombinant MaIDH has a broader pH optimum from pH 6.5 to pH 8.5 Microcystis aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 5.5 9.5 activity range Microcystis aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+ purified recombinant MaIDH is completely specific with NADP+, and no NAD+-dependent activity is detectable Microcystis aeruginosa
1.1.1.42 NADP+ the enzyme is completely NADP+-dependent and no NAD+-linked activity is detectable Microcystis aeruginosa
1.1.1.42 NADPH
-
 Microcystis aeruginosa

General Information

EC Number General Information Comment Organism
1.1.1.42 physiological function isocitrate dehydrogenase of Microcystis aeruginosa plays important roles in energy and biosynthesis metabolisms and its catalytic product 2-oxoglutarate provides the carbon skeleton for ammonium assimilation and also constitutes a signaling molecule of nitrogen starvation in cyanobacteria Microcystis aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.42 0.00335
-
 DL-isocitrate with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.00348
-
 DL-isocitrate with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.00613
-
 NADP+ with Mn2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 0.00787
-
 NADP+ with Mg2+, pH 7.5, 50°C Microcystis aeruginosa
1.1.1.42 335
-
 DL-isocitrate with Mg2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 348
-
 DL-isocitrate with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 613
-
 NADP+ with Mn2+, pH 7.5, 37°C Microcystis aeruginosa
1.1.1.42 787
-
 NADP+ with Mg2+, pH 7.5, 37°C Microcystis aeruginosa