BRENDA - Enzyme Database

Expression and characterization of a novel isocitrate dehydrogenase from Streptomyces diastaticus No. 7 strain M1033

Zhang, B.; Wang, P.; Wang, A.; Wang, W.; Tang, W.; Zhu, G.; Mol. Biol. Rep. 40, 1615-1623 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
SdIDH, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)
Streptomyces diastaticus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
Ca2+
-
Streptomyces diastaticus
1.1.1.42
Co2+
-
Streptomyces diastaticus
1.1.1.42
Cu2+
complete inhibition
Streptomyces diastaticus
1.1.1.42
EDTA
-
Streptomyces diastaticus
1.1.1.42
K+
-
Streptomyces diastaticus
1.1.1.42
Na+
-
Streptomyces diastaticus
1.1.1.42
Zn2+
-
Streptomyces diastaticus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
activates, about 50% activity compared to Mn2+, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively
Streptomyces diastaticus
1.1.1.42
Mn2+
activates, preferred cation, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively
Streptomyces diastaticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
70000
-
gel filtration
Streptomyces diastaticus
1.1.1.42
80000
-
1 * 80000, SDS-PAGE
Streptomyces diastaticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
Streptomyces diastaticus
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
DL-isocitrate + NADP+
Streptomyces diastaticus M1033
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Streptomyces diastaticus
-
-
-
1.1.1.42
Streptomyces diastaticus M1033
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3)
Streptomyces diastaticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NAD+
low activityy with NAD+
723151
Streptomyces diastaticus
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NAD+
low activityy with NAD+
723151
Streptomyces diastaticus M1033
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
-
723151
Streptomyces diastaticus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
NADP+ is the highly preferred cofactor
723151
Streptomyces diastaticus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
-
723151
Streptomyces diastaticus M1033
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
NADP+ is the highly preferred cofactor
723151
Streptomyces diastaticus M1033
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
monomer
1 * 80000, SDS-PAGE
Streptomyces diastaticus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
37
-
with Mg2+
Streptomyces diastaticus
1.1.1.42
40
-
with Mn2+
Streptomyces diastaticus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.42
25
45
activity range, profile overview
Streptomyces diastaticus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
40
-
purified enzyme, 20 min, stable up to 40°C
Streptomyces diastaticus
1.1.1.42
47
-
purified enzyme, 20 min, loss of 50% activity
Streptomyces diastaticus
1.1.1.42
50
-
purified enzyme, 20 min, inactivation
Streptomyces diastaticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8
-
with Mn2+
Streptomyces diastaticus
1.1.1.42
9
-
with Mg2+
Streptomyces diastaticus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
7
11
activity range, profile overview
Streptomyces diastaticus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
additional information
SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively
Streptomyces diastaticus
1.1.1.42
NAD+
poor cofactor
Streptomyces diastaticus
1.1.1.42
NADP+
dependent on
Streptomyces diastaticus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
SdIDH, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)
Streptomyces diastaticus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
additional information
SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively
Streptomyces diastaticus
1.1.1.42
NAD+
poor cofactor
Streptomyces diastaticus
1.1.1.42
NADP+
dependent on
Streptomyces diastaticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
Ca2+
-
Streptomyces diastaticus
1.1.1.42
Co2+
-
Streptomyces diastaticus
1.1.1.42
Cu2+
complete inhibition
Streptomyces diastaticus
1.1.1.42
EDTA
-
Streptomyces diastaticus
1.1.1.42
K+
-
Streptomyces diastaticus
1.1.1.42
Na+
-
Streptomyces diastaticus
1.1.1.42
Zn2+
-
Streptomyces diastaticus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
activates, about 50% activity compared to Mn2+, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively
Streptomyces diastaticus
1.1.1.42
Mn2+
activates, preferred cation, SdIDH displays a 19000-32000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ and Mg2+, respectively
Streptomyces diastaticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
70000
-
gel filtration
Streptomyces diastaticus
1.1.1.42
80000
-
1 * 80000, SDS-PAGE
Streptomyces diastaticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
Streptomyces diastaticus
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
1.1.1.42
DL-isocitrate + NADP+
Streptomyces diastaticus M1033
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3)
Streptomyces diastaticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NAD+
low activityy with NAD+
723151
Streptomyces diastaticus
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NAD+
low activityy with NAD+
723151
Streptomyces diastaticus M1033
2-oxoglutarate + CO2 + NADH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
-
723151
Streptomyces diastaticus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
NADP+ is the highly preferred cofactor
723151
Streptomyces diastaticus
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
-
723151
Streptomyces diastaticus M1033
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
NADP+ is the highly preferred cofactor
723151
Streptomyces diastaticus M1033
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
monomer
1 * 80000, SDS-PAGE
Streptomyces diastaticus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
37
-
with Mg2+
Streptomyces diastaticus
1.1.1.42
40
-
with Mn2+
Streptomyces diastaticus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.42
25
45
activity range, profile overview
Streptomyces diastaticus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
40
-
purified enzyme, 20 min, stable up to 40°C
Streptomyces diastaticus
1.1.1.42
47
-
purified enzyme, 20 min, loss of 50% activity
Streptomyces diastaticus
1.1.1.42
50
-
purified enzyme, 20 min, inactivation
Streptomyces diastaticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8
-
with Mn2+
Streptomyces diastaticus
1.1.1.42
9
-
with Mg2+
Streptomyces diastaticus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
7
11
activity range, profile overview
Streptomyces diastaticus
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
metabolism
NAD(P)+-dependent isocitrate dehydrogenase is a key enzyme in tricarboxylic acid cycle
Streptomyces diastaticus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
metabolism
NAD(P)+-dependent isocitrate dehydrogenase is a key enzyme in tricarboxylic acid cycle
Streptomyces diastaticus