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Literature summary extracted from

  • Wang, Z.; Wang, B.; Ge, Y.; Pan, W.; Wang, J.; Xu, L.; Liu, A.; Zhu, G.
    Expression and identification of a thermostable malate dehydrogenase from multicellular prokaryote Streptomyces avermitilis MA-4680 (2011), Mol. Biol. Rep., 38, 1629-1636.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.37 biotechnology MDH is widely used in coenzyme regeneration, antigen immunoassays and bioreactors Streptomyces avermitilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 sequence comparisons, recombinant expression of His-tagged MDH in Escherichia coli strain Rosetta (DE3) Streptomyces avermitilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 Hg2+
-
 Streptomyces avermitilis
1.1.1.37 Mn2+
-
 Streptomyces avermitilis
1.1.1.37 Ni2+
-
 Streptomyces avermitilis
1.1.1.37 oxaloacetate substrate inhibition Streptomyces avermitilis
1.1.1.37 Zn2+
-
 Streptomyces avermitilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.37 Ca2+ activates Streptomyces avermitilis
1.1.1.37 Cu2+ activates Streptomyces avermitilis
1.1.1.37 Mg2+ activates Streptomyces avermitilis
1.1.1.37 additional information Co2+ has no effect on the activity Streptomyces avermitilis
1.1.1.37 Na+ activates Streptomyces avermitilis
1.1.1.37 NH4+ activates Streptomyces avermitilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 34689
-
 x * 35000, SDS-PAGE, x * 34689, sequence calculation Streptomyces avermitilis
1.1.1.37 35000
-
 x * 35000, SDS-PAGE, x * 34689, sequence calculation Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Streptomyces avermitilis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Streptomyces avermitilis MA-4680
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Streptomyces avermitilis
-
-
-
1.1.1.37 Streptomyces avermitilis MA-4680
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Streptomyces avermitilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ 1246fold preference for oxaloacetate reduction over L-malate oxidation Streptomyces avermitilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Streptomyces avermitilis MA-4680 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ 1246fold preference for oxaloacetate reduction over L-malate oxidation Streptomyces avermitilis MA-4680 oxaloacetate + NADH + H+
-
 r
1.1.1.37 additional information the recombinant SaMDH may also use NADPH as a cofactor although it is a highly NAD(H)-specific enzyme. No activity with malate and NADP+ as substrates Streptomyces avermitilis ?
-
 ?
1.1.1.37 additional information the recombinant SaMDH may also use NADPH as a cofactor although it is a highly NAD(H)-specific enzyme. No activity with malate and NADP+ as substrates Streptomyces avermitilis MA-4680 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 ? x * 35000, SDS-PAGE, x * 34689, sequence calculation Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 MDH
-
 Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 42
-
 recombinant enzyme Streptomyces avermitilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.37 20 60 activity range, profile overview Streptomyces avermitilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 50
-
 purified recombinant enzyme, half-life is 160 min, recombinant enzyme Streptomyces avermitilis
1.1.1.37 55
-
 purified recombinant enzyme, stable below 55°C, loss of 18% activity after 10 min Streptomyces avermitilis
1.1.1.37 60
-
 purified recombinant enzyme, loss of 47% activity after 10 min Streptomyces avermitilis
1.1.1.37 65
-
 purified recombinant enzyme, loss of 80% activity after 10 min Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 8
-
 recombinant enzyme Streptomyces avermitilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.37 7.5 9 activity range Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 additional information the recombinant SaMDH may also use NADPH as a cofactor although it is a highly NAD(H)-specific enzyme, no activity with malate and NADP+ as substrates Streptomyces avermitilis
1.1.1.37 NAD+
-
 Streptomyces avermitilis
1.1.1.37 NADH
-
 Streptomyces avermitilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.37 1.27
-
 oxaloacetate pH 8.0, 42°C Streptomyces avermitilis