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Literature summary extracted from

  • Murata, D.; Nomura, K.H.; Dejima, K.; Mizuguchi, S.; Kawasaki, N.; Matsuishi-Nakajima, Y.; Ito, S.; Gengyo-Ando, K.; Kage-Nakadai, E.; Mitani, S.; Nomura, K.
    GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans (2012), Mol. Biol. Cell, 23, 982-995.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.198 genes involved in GPI-anchor synthesis in Caenorhabditis elegans, overview. Isolation and analysis of piga-1-knockout allele tm2939 Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.198 additional information the RNAi phenotypes of each gene, including larval arrest, scrawny larvae, and germline defects, may result from N-/O-glycosylation inhibition as well as GPI-anchor inhibition Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.198 UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol Caenorhabditis elegans
-
 UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.198 Caenorhabditis elegans
-
 gene piga-1 encoding the catalytic subunit of the phosphatidylinositol N-acetylglucosaminyltransferase complex
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.198 germ cell
-
 Caenorhabditis elegans
-
2.4.1.198 gonad
-
 Caenorhabditis elegans
-
2.4.1.198 additional information the wild-type PIGA-1 protein is solely detected in the germline, and no EGFP-tagged PIGA-1 is detected in so­matic cells Caenorhabditis elegans
-
2.4.1.198 muscle
-
 Caenorhabditis elegans
-
2.4.1.198 neuron
-
 Caenorhabditis elegans
-
2.4.1.198 oocyte
-
 Caenorhabditis elegans
-
2.4.1.198 sperm
-
 Caenorhabditis elegans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.198 UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
-
 Caenorhabditis elegans UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.198 glycosylphosphatidylinositol N-acetylglucosaminyltrans­ferase complex
-
 Caenorhabditis elegans
2.4.1.198 GPI-GnT
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
2.4.1.198 malfunction piga-1-knockout worms show 100% lethality, with decreased mitotic germline cells and abnormal eggshell formation. Cell-specific rescue of the null allele, expression of piga-1 in somatic gonads and/or in germline is sufficient for normal embryonic development and the maintenance of the germline mitotic cells. The RNAi phenotypes of each gene, including larval arrest, scrawny larvae, and germline defects, may result from N-/O-glycosylation inhibition as well as GPI-anchor inhibition Caenorhabditis elegans
2.4.1.198 physiological function the phosphatidylinositol N-acetylglucosaminyltransferase complex catalyzes the first step of GPI-anchor syn­thesis, which is indispensable for the germline development of the nematode Caenorhabditis elegans. GPI-anchor synthesis is indispens­able for the maintenance of mitotic germline cell number Caenorhabditis elegans