EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.106 | expression in Escherichia coli | Aspergillus fumigatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.106 | Mg2+ | at 2-5 mM, slight activation. Inhibitory above 10 mM | Aspergillus fumigatus | |
2.5.1.106 | Mn2+ | inhibitory above 5 mM | Aspergillus fumigatus | |
2.5.1.106 | additional information | not inhibitory: EDTA up to 5 mM | Aspergillus fumigatus | |
2.5.1.106 | Zn2+ | inhibitory above 5 mM | Aspergillus fumigatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.106 | 0.055 | - |
brevianamide F | pH 7.5, 30°C | Aspergillus fumigatus | |
2.5.1.106 | 0.074 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Aspergillus fumigatus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.106 | Ca2+ | at 2-5 mM, slight activation | Aspergillus fumigatus | |
2.5.1.106 | Mg2+ | at 2-5 mM, slight activation. Inhibitory above 10 mM | Aspergillus fumigatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.106 | 49000 | - |
1 * 49000, SDS-PAGE, 1 * 53400, calculated | Aspergillus fumigatus |
2.5.1.106 | 53400 | - |
1 * 49000, SDS-PAGE, 1 * 53400, calculated | Aspergillus fumigatus |
2.5.1.106 | 66000 | - |
gel filtration | Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.106 | Aspergillus fumigatus | Q4WAW7 | - |
- |
2.5.1.106 | Aspergillus fumigatus Af293 | Q4WAW7 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.106 | dimethylallyl diphosphate + brevianamide F | catalyzes the prenylation of cyclo-L-Trp-L-Pro , i.e.brevianamide F, at the C-2 position of the indole nucleus | Aspergillus fumigatus | diphosphate + tryprostatin B | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + brevianamide F | catalyzes the prenylation of cyclo-L-Trp-L-Pro , i.e.brevianamide F, at the C-2 position of the indole nucleus | Aspergillus fumigatus Af293 | diphosphate + tryprostatin B | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro) | 32.6% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Pro) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro) | 32.6% of the activity with brevianamide F | Aspergillus fumigatus Af293 | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Pro) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-Gly) | 4.7% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-Gly) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-Gly) | 4.7% of the activity with brevianamide F | Aspergillus fumigatus Af293 | diphosphate + cyclo-(L-2-dimethylallyl-Trp-Gly) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-L-Leu) | 21.9% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Leu) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-L-Leu) | 21.9% of the activity with brevianamide F | Aspergillus fumigatus Af293 | diphosphate + cyclo-(D-2-dimethylallyl-Trp-L-Leu) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-L-Phe) | 3.4% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Phe) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-L-Trp) | 6.4% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Trp) | - |
? | |
2.5.1.106 | dimethylallyl diphosphate + cyclo-(L-Trp-L-Tyr) | 5.0% of the activity with brevianamide F | Aspergillus fumigatus | diphosphate + cyclo-(L-2-dimethylallyl-Trp-L-Tyr) | - |
? | |
2.5.1.106 | additional information | enzyme shows a high substrate specificity towards dimethylallyl diphosphate, but accepts different tryptophan-containing cyclic dipeptides. No substrates: isopentenyl diphosphate or geranyl diphosphate | Aspergillus fumigatus | ? | - |
? | |
2.5.1.106 | additional information | enzyme shows a high substrate specificity towards dimethylallyl diphosphate, but accepts different tryptophan-containing cyclic dipeptides. No substrates: isopentenyl diphosphate or geranyl diphosphate | Aspergillus fumigatus Af293 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.106 | monomer | 1 * 49000, SDS-PAGE, 1 * 53400, calculated | Aspergillus fumigatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.106 | FtmPT1 | - |
Aspergillus fumigatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.106 | 5.57 | - |
brevianamide F | pH 7.5, 30°C | Aspergillus fumigatus |