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Literature summary extracted from

  • Jariyachawalid, K.; Laowanapiban, P.; Meevootisom, V.; Wiyakrutta, S.
    Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES (2012), Microb. Cell Fact., 11, 47.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.72 synthesis functional expression in Pichia pastoris. Co-expression of Escherichia coli chaperonins GroEL-GroES with the enzyme gene dramatically improves the soluble active enzyme production. Increasing gene dosage of both the enzyme and those of the chaperones further increases functional protein yield up to 14400fold higher than when the enzyme is expressed alone. Optimization of cultivation condition further increases activity yield from the best co-expressing strain by 1.2fold Stutzerimonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.72 Stutzerimonas stutzeri Q6VY99
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2.6.1.72 Stutzerimonas stutzeri ST201 Q6VY99
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-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.72 D-PhgAT
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 Stutzerimonas stutzeri
2.6.1.72 dpgA
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 Stutzerimonas stutzeri