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Literature summary extracted from

  • Bartolucci, S.; de Pascale, D.; Rossi, M.
    Protein disulfide oxidoreductase from Pyrococcus furiosus: biochemical properties (2001), Methods Enzymol., 334, 62-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.8 expression in Escherichia coli Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.8 25649
-
 x * 25649, electrospray mass spectrometry Pyrococcus furiosus
1.8.1.8 26000
-
 x * 26000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.8 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.8
-
 Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
1.8.1.8 -20°C, stable fo at least 3 months without any loss of activity Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.8 insulin disulfide + NADPH
-
 Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.8 ? x * 26000, SDS-PAGE Pyrococcus furiosus
1.8.1.8 ? x * 25649, electrospray mass spectrometry Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.8 90
-
 3 h, no loss of activity Pyrococcus furiosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.8 Pyrococcus furiosus isoelectric focusing
-
 4.9