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Literature summary extracted from

  • Ohashi-Suzuki, M.; Yabu, Y.; Ohshima, S.; Nakamura, K.; Kido, Y.; Sakamoto, K.; Kita, K.; Ohta, N.; Suzuki, T.
    Differential kinetic activities of glycerol kinase among African trypanosome species: phylogenetic and therapeutic implications (2011), J. Vet. Med. Sci., 73, 615-621.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.30 expressed in Escherichia coli Trypanosoma brucei brucei
2.7.1.30 expressed in Escherichia coli Trypanosoma vivax
2.7.1.30 expressed in Escherichia coli Trypanosoma congolense

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.30 glycerol inhibitor of the glycerol kinase reverse reaction Trypanosoma brucei brucei
2.7.1.30 glycerol inhibitor of the glycerol kinase reverse reaction Trypanosoma congolense
2.7.1.30 glycerol inhibitor of the glycerol kinase reverse reaction Trypanosoma vivax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.30 0.24
-
 ADP in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma congolense
2.7.1.30 0.37
-
 ADP in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma brucei brucei
2.7.1.30 0.69
-
 ADP in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma vivax
2.7.1.30 1.09
-
 sn-glycerol 3-phosphate in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma congolense
2.7.1.30 1.13
-
 sn-glycerol 3-phosphate in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma brucei brucei
2.7.1.30 1.37
-
 sn-glycerol 3-phosphate in 0.1 M MOPS buffer (pH 6.8) containing 1 mM EDTA, 5 mM MgSO4, temperature not specified in the publication Trypanosoma vivax

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.30 glycosome
-
 Trypanosoma brucei brucei 20015
-
2.7.1.30 glycosome
-
 Trypanosoma vivax 20015
-
2.7.1.30 glycosome
-
 Trypanosoma congolense 20015
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 50000
-
 x * 50000, SDS-PAGE Trypanosoma brucei brucei
2.7.1.30 50000
-
 x * 50000, SDS-PAGE Trypanosoma vivax
2.7.1.30 50000
-
 x * 50000, SDS-PAGE Trypanosoma congolense
2.7.1.30 54600
-
 x * 54600, calculated from amino acid sequence Trypanosoma vivax
2.7.1.30 56200
-
 x * 56200, calculated from amino acid sequence Trypanosoma congolense

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Trypanosoma brucei brucei Q9NJP9
-
-
2.7.1.30 Trypanosoma brucei brucei ILTat1.4 Q9NJP9
-
-
2.7.1.30 Trypanosoma congolense Q75T26
-
-
2.7.1.30 Trypanosoma congolense IL1180 Q75T26
-
-
2.7.1.30 Trypanosoma vivax B0I530
-
-
2.7.1.30 Trypanosoma vivax IL1392 B0I530
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30 nickel affinity column chromatography Trypanosoma brucei brucei
2.7.1.30 nickel affinity column chromatography Trypanosoma vivax
2.7.1.30 nickel affinity column chromatography Trypanosoma congolense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma brucei brucei ATP + glycerol
-
 r
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma vivax ATP + glycerol
-
 r
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma congolense ATP + glycerol
-
 r
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma congolense IL1180 ATP + glycerol
-
 r
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma brucei brucei ILTat1.4 ATP + glycerol
-
 r
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma vivax IL1392 ATP + glycerol
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma brucei brucei ADP + sn-glycerol 3-phosphate
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma vivax ADP + sn-glycerol 3-phosphate
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma congolense ADP + sn-glycerol 3-phosphate
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma congolense IL1180 ADP + sn-glycerol 3-phosphate
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma brucei brucei ILTat1.4 ADP + sn-glycerol 3-phosphate
-
 r
2.7.1.30 ATP + glycerol
-
 Trypanosoma vivax IL1392 ADP + sn-glycerol 3-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.30 ? x * 50000, SDS-PAGE Trypanosoma brucei brucei
2.7.1.30 ? x * 50000, SDS-PAGE Trypanosoma vivax
2.7.1.30 ? x * 50000, SDS-PAGE Trypanosoma congolense
2.7.1.30 ? x * 56200, calculated from amino acid sequence Trypanosoma congolense
2.7.1.30 ? x * 54600, calculated from amino acid sequence Trypanosoma vivax