Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Neily, M.H.; Matsukura, C.; Maucourt, M.; Bernillon, S.; Deborde, C.; Moing, A.; Yin, Y.G.; Saito, T.; Mori, K.; Asamizu, E.; Rolin, D.; Moriguchi, T.; Ezura, H.
    Enhanced polyamine accumulation alters carotenoid metabolism at the transcriptional level in tomato fruit over-expressing spermidine synthase (2011), J. Plant Physiol., 168, 242-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 gene SPDS1, functional overexpression in transgenic Solanum lycopersicum cv. Micro-Tom plants via the Rhizobium radiobactor, strain GV2260, or Agrobacterium tumefaciens-mediated transformation method, quantitative real-time PCR enzyme expression analysis Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosylmethioninamine + putrescine Malus domestica
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Malus domestica Q8GTQ6 gene SPDS1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosylmethioninamine + putrescine
-
 Malus domestica S-methyl-5'-thioadenosine + spermidine
-
 ?

General Information

EC Number General Information Comment Organism
2.5.1.16 metabolism spermidine synthase is a key enzyme for polyamine biosynthesis Malus domestica
2.5.1.16 additional information homozygous overexpressing transgenic tomato plant lines show a high level of Md-SPDS1 mRNA expression and a 1.5 to 2fold increase in the levels of free spermidine in fruits compared to the wild-type plants. Analysis of pericarp-columella and metabolites in placenta tissues reveal distinct metabolic profiles between the wild-type and transgenic lines, particularly at the late ripening stages, phenotypes, overview. The transgenic tomato fruits also show an increase in carotenoid accumulation, especially in lycopene, and increased ethylene production compared to wild-type fruits. Genes responsible for lycopene biosynthesis, including phytoene synthase, phytoene desaturase, and deoxy-D-xylulose 5-phosphate synthase, are significantly up-regulated in ripe transgenic fruits, whereas genes involved in lycopene degradation, including lycopene-epsilon cyclase and lycopene beta cyclase, are downregulated in the transgenic fruits compared to the wild-type Malus domestica