BRENDA - Enzyme Database

Kinetic and expression profiling of cytosolic pyruvate kinase enzyme during seed development of Indian mustard (Brassica juncea)

Mandal, S.; Bose, P.; Dawar, S.; Rajarani, A.; Santha, I.; J. Plant Biochem. Biotechnol. 20, 102-109 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.40
expression profiling by RT-PCR techniques
Brassica juncea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
3-phosphoglycerate
8% inhibition at 5 mM
Brassica juncea
2.7.1.40
AMP
11% inhibition at 5 mM
Brassica juncea
2.7.1.40
ATP
48% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme
Brassica juncea
2.7.1.40
citrate
60% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme
Brassica juncea
2.7.1.40
isocitrate
30% inhibition at 5 mM
Brassica juncea
2.7.1.40
additional information
poor inhibition of glucose 1-phosphate and fumaric acid at 5 mM
Brassica juncea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
additional information
-
additional information
kinetics, in presence of saturated substrate concentration, the enzyme exhibits hyperbolic kinetics for both ADP and PEP
Brassica juncea
2.7.1.40
0.0965
-
ADP
pH 7.0, 25°C
Brassica juncea
2.7.1.40
0.1785
-
phosphoenolpyruvate
pH 7.0, 25°C
Brassica juncea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
chloroplast
plastidial isozyme
Brassica juncea
9507
-
2.7.1.40
cytoplasm
cytosolic isozyme
Brassica juncea
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
Mg2+
required
Brassica juncea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.40
ATP + pyruvate
Brassica juncea
-
ADP + phosphoenolpyruvate
-
-
r
2.7.1.40
ATP + pyruvate
Brassica juncea Pusa Bold
-
ADP + phosphoenolpyruvate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Brassica juncea
-
-
-
2.7.1.40
Brassica juncea Pusa Bold
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
native cytosolic isozyme 12.3fold from seeds by anion exchange and hydroxyapatite chromatography, inactivation of plastidial isoform by heating the enzyme extract at 60°C for 3 min
Brassica juncea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
flower
-
Brassica juncea
-
2.7.1.40
leaf
-
Brassica juncea
-
2.7.1.40
seed
expression profiling of cytosolic pyruvate kinase enzyme during seed development, enzyme activity is highest during middle stage, overview
Brassica juncea
-
2.7.1.40
seedling
highest activity
Brassica juncea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.40
690.7
-
partially purified cytosolic isozyme, pH 7.0, 25°C
Brassica juncea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ATP + pyruvate
-
723044
Brassica juncea
ADP + phosphoenolpyruvate
-
-
-
r
2.7.1.40
ATP + pyruvate
-
723044
Brassica juncea Pusa Bold
ADP + phosphoenolpyruvate
-
-
-
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.40
25
-
assay at
Brassica juncea
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.40
60
-
3 min, pH 7.0, plastidial isoform, inactivation
Brassica juncea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
7
-
narrow optimum, cytosolic isozyme
Brassica juncea
2.7.1.40
8
-
plastidial isozyme
Brassica juncea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
ATP
-
Brassica juncea
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
3.64
-
pH 7.0, 25°C
Brassica juncea
citrate
2.7.1.40
5
-
pH 7.0, 25°C
Brassica juncea
ATP
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
expression profiling by RT-PCR techniques
Brassica juncea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
ATP
-
Brassica juncea
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
3.64
-
pH 7.0, 25°C
Brassica juncea
citrate
2.7.1.40
5
-
pH 7.0, 25°C
Brassica juncea
ATP
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
3-phosphoglycerate
8% inhibition at 5 mM
Brassica juncea
2.7.1.40
AMP
11% inhibition at 5 mM
Brassica juncea
2.7.1.40
ATP
48% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme
Brassica juncea
2.7.1.40
citrate
60% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme
Brassica juncea
2.7.1.40
isocitrate
30% inhibition at 5 mM
Brassica juncea
2.7.1.40
additional information
poor inhibition of glucose 1-phosphate and fumaric acid at 5 mM
Brassica juncea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
additional information
-
additional information
kinetics, in presence of saturated substrate concentration, the enzyme exhibits hyperbolic kinetics for both ADP and PEP
Brassica juncea
2.7.1.40
0.0965
-
ADP
pH 7.0, 25°C
Brassica juncea
2.7.1.40
0.1785
-
phosphoenolpyruvate
pH 7.0, 25°C
Brassica juncea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
chloroplast
plastidial isozyme
Brassica juncea
9507
-
2.7.1.40
cytoplasm
cytosolic isozyme
Brassica juncea
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
Mg2+
required
Brassica juncea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.40
ATP + pyruvate
Brassica juncea
-
ADP + phosphoenolpyruvate
-
-
r
2.7.1.40
ATP + pyruvate
Brassica juncea Pusa Bold
-
ADP + phosphoenolpyruvate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
native cytosolic isozyme 12.3fold from seeds by anion exchange and hydroxyapatite chromatography, inactivation of plastidial isoform by heating the enzyme extract at 60°C for 3 min
Brassica juncea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
flower
-
Brassica juncea
-
2.7.1.40
leaf
-
Brassica juncea
-
2.7.1.40
seed
expression profiling of cytosolic pyruvate kinase enzyme during seed development, enzyme activity is highest during middle stage, overview
Brassica juncea
-
2.7.1.40
seedling
highest activity
Brassica juncea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.40
690.7
-
partially purified cytosolic isozyme, pH 7.0, 25°C
Brassica juncea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ATP + pyruvate
-
723044
Brassica juncea
ADP + phosphoenolpyruvate
-
-
-
r
2.7.1.40
ATP + pyruvate
-
723044
Brassica juncea Pusa Bold
ADP + phosphoenolpyruvate
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.40
25
-
assay at
Brassica juncea
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.40
60
-
3 min, pH 7.0, plastidial isoform, inactivation
Brassica juncea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
7
-
narrow optimum, cytosolic isozyme
Brassica juncea
2.7.1.40
8
-
plastidial isozyme
Brassica juncea
General Information
EC Number
General Information
Commentary
Organism
2.7.1.40
metabolism
pyruvate kinase is a crucial regulatory enzyme involved in glycolysis
Brassica juncea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.40
metabolism
pyruvate kinase is a crucial regulatory enzyme involved in glycolysis
Brassica juncea