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Literature summary extracted from

  • Siow, R.; Teo, S.; Ho, W.; Shukor, M.; Phang, S.; Ho, C.
    Molecular cloning and biochemical characterization of galactose-1-phosphate uridylyltransferase from Gracilaria changii (Rhodophyta) (2012), J. Phycol., 48, 155-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.12 gene GALT, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21(DE3) plysS Gracilaria changii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 additional information
-
 additional information kinetics forward and reverse reaction, overview Gracilaria changii
2.7.7.12 0.051
-
 UDP-alpha-D-galactose pH 7.5, 25°C Gracilaria changii
2.7.7.12 0.092
-
 alpha-D-glucose 1-phosphate pH 7.5, 25°C Gracilaria changii
2.7.7.12 0.116
-
 alpha-D-galactose 1-phosphate pH 7.5, 25°C Gracilaria changii
2.7.7.12 0.134
-
 UDP-alpha-D-glucose pH 7.5, 25°C Gracilaria changii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.12 Fe2+ required, enzyme-bound Gracilaria changii
2.7.7.12 Mg2+ required Gracilaria changii
2.7.7.12 Zn2+ required, enzyme-bound Gracilaria changii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Gracilaria changii
-
 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Gracilaria changii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 recombinant enzyme from Escherichia coli strain BL21(DE3) plysS Gracilaria changii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 Gracilaria changii UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 r
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Gracilaria changii alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 More homology modeling of the three-dimensional protein structure, overview Gracilaria changii

Synonyms

EC Number Synonyms Comment Organism
2.7.7.12 galactose-1-phosphate uridylyltransferase
-
 Gracilaria changii
2.7.7.12 GALT
-
 Gracilaria changii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.12 25
-
 assay at Gracilaria changii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 7.5
-
 assay at Gracilaria changii

General Information

EC Number General Information Comment Organism
2.7.7.12 additional information homology modeling of the three-dimensional protein structure, overview Gracilaria changii