EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.2 | expression in Escherichia coli | Parageobacillus thermoglucosidasius |
2.4.2.2 | expression in Escherichia coli | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.2 | 0.15 | - |
uridine | pH 7.0, 60°C | Thermus thermophilus | |
2.4.2.2 | 0.43 | - |
thymidine | pH 7.0, 60°C | Thermus thermophilus | |
2.4.2.2 | 1.3 | - |
thymidine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius | |
2.4.2.2 | 2.3 | - |
uridine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.2 | Parageobacillus thermoglucosidasius | - |
- |
- |
2.4.2.2 | Thermus thermophilus | Q72HS4 | - |
- |
2.4.2.2 | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72HS4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.2 | (2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate | 1.6% substrate conversion after 30 min | ? | |
2.4.2.2 | (2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate | 1.6% substrate conversion after 30 min | ? | |
2.4.2.2 | 2'-deoxy-2'-fluorouridine + phosphate | - |
Parageobacillus thermoglucosidasius | ? | poor activity, 0.4% substrate conversion after 30 min | ? | |
2.4.2.2 | 2'-deoxy-2'-fluorouridine + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate | 15.6% substrate conversion after 30 min | ? | |
2.4.2.2 | 2'-deoxy-2'-fluorouridine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate | 15.6% substrate conversion after 30 min | ? | |
2.4.2.2 | thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | thymidine + phosphate | - |
Thermus thermophilus | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | thymidine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | uridine + phosphate | prefered substrate | Parageobacillus thermoglucosidasius | uracil + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | uridine + phosphate | prefered substrate | Thermus thermophilus | uracil + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | uridine + phosphate | prefered substrate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | uracil + alpha-D-ribose 1-phosphate | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.2 | 70 | - |
half-life 1.6 h | Parageobacillus thermoglucosidasius |
2.4.2.2 | 70 | - |
half-life 1.6 h | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.2 | 83 | - |
thymidine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius | |
2.4.2.2 | 277 | - |
uridine | pH 7.0, 60°C | Parageobacillus thermoglucosidasius | |
2.4.2.2 | 277 | - |
uridine | pH 7.0, 60°C | Thermus thermophilus | |
2.4.2.2 | 679 | - |
thymidine | pH 7.0, 60°C | Thermus thermophilus |