EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.30 | expressed in Escherichia coli BL21(DE3) | Mesorhizobium loti |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.30 | M2T/V70K | M2: surface residue | Mesorhizobium loti |
2.6.1.30 | M2T/V70K/E212G | E212: surface residue | Mesorhizobium loti |
2.6.1.30 | M2T/Y35H/V70K | Y35: active site residur | Mesorhizobium loti |
2.6.1.30 | M2T/Y35H/V70K/E212G | E212: surface residue | Mesorhizobium loti |
2.6.1.30 | V70K | active site residue, increase in reactivity toward 2-oxoglutarate | Mesorhizobium loti |
2.6.1.30 | V70K/E212G | E212: surface residue | Mesorhizobium loti |
2.6.1.30 | V70R | active site residue, elimination of activity | Mesorhizobium loti |
2.6.1.30 | V70R/F247C | F247: hydrophobic core residue | Mesorhizobium loti |
2.6.1.30 | Y35H/V70K | active site residues | Mesorhizobium loti |
2.6.1.30 | Y35H/V70R/F247C | F247: hydrophobic core residue | Mesorhizobium loti |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.30 | 8.8 | - |
2-oxoglutarate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 12.6 | - |
2-oxoglutarate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 17.2 | - |
2-oxoglutarate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 21.1 | - |
2-oxoglutarate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 29.3 | - |
L-glutamate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 42.4 | - |
L-glutamate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 44.2 | - |
L-glutamate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 51 | - |
L-glutamate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 61.3 | - |
2-oxoglutarate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 77.3 | - |
2-oxoglutarate | M2T/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 80.1 | - |
2-oxoglutarate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 102.6 | - |
2-oxoglutarate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 106.8 | - |
2-oxoglutarate | M2T/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 110.3 | - |
2-oxoglutarate | V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 123.4 | - |
L-glutamate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 133.5 | - |
L-glutamate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 142 | - |
L-glutamate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.30 | 41000 | - |
4 * 41000, SDS-PAGE, gel filtration | Mesorhizobium loti |
2.6.1.30 | 154000 | - |
gel filtration | Mesorhizobium loti |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.30 | Mesorhizobium loti | Q988B8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.30 | hydrophobic interaction chromatography, gel filtration | Mesorhizobium loti |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.30 | pyridoxamine + 2-oxoglutarate | - |
Mesorhizobium loti | pyridoxal + L-glutamate | - |
r | |
2.6.1.30 | pyridoxamine + pyruvate | - |
Mesorhizobium loti | pyridoxal + L-alanine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.30 | homotetramer | 4 * 41000, SDS-PAGE, gel filtration | Mesorhizobium loti |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.30 | pyridoxamine-pyruvate aminotransferase | pyridoxamine-2-oxoglutarate aminotransferase activity | Mesorhizobium loti |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.30 | 1.5 | - |
L-glutamate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 1.9 | - |
2-oxoglutarate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 2.7 | - |
L-glutamate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 2.9 | - |
L-glutamate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 3 | - |
L-glutamate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 3.1 | - |
L-glutamate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 3.5 | - |
2-oxoglutarate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 3.9 | - |
2-oxoglutarate | M2T/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 4 | - |
2-oxoglutarate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 4.1 | - |
2-oxoglutarate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 4.4 | - |
L-glutamate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 4.6 | - |
2-oxoglutarate | M2T/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 4.6 | - |
2-oxoglutarate | V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 5.2 | - |
2-oxoglutarate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 6.4 | - |
L-glutamate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 8.1 | - |
2-oxoglutarate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 9.1 | - |
2-oxoglutarate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.30 | 0.012 | - |
L-glutamate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.019 | - |
L-glutamate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.023 | - |
2-oxoglutarate | wild type protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.033 | - |
L-glutamate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.04 | - |
2-oxoglutarate | V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.042 | - |
2-oxoglutarate | V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.043 | - |
2-oxoglutarate | M2T/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.05 | - |
2-oxoglutarate | M2T/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.058 | - |
L-glutamate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.07 | - |
L-glutamate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.1 | - |
L-glutamate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.148 | - |
2-oxoglutarate | V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.152 | - |
L-glutamate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.167 | - |
2-oxoglutarate | M2T/Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.234 | - |
2-oxoglutarate | M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.408 | - |
2-oxoglutarate | Y35H/V70K mutant protein, pH 9.0, 30°C | Mesorhizobium loti | |
2.6.1.30 | 0.916 | - |
2-oxoglutarate | Y35H/V70R/F247C mutant protein, pH 9.0, 30°C | Mesorhizobium loti |