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Literature summary extracted from

  • Hong, E.; Cha, M.; Yun, H.; Kim, B.
    Asymmetric synthesis of L-tert-leucine and L-3-hydroxyadamantylglycine using branched chain aminotransferase (2010), J. Mol. Catal. B, 66, 228-233.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 His-tagged protein expressed in Escherichia coli BL21 Escherichia coli
2.6.1.42 His-tagged protein expressed in Escherichia coli BL21 Enterobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 2-oxoglutarate
-
Enterobacter sp.
2.6.1.42 2-oxoglutarate
-
Escherichia coli
2.6.1.42 aminooxyacetic acid
-
Enterobacter sp.
2.6.1.42 gabaculine
-
Enterobacter sp.
2.6.1.42 hydroxylamine
-
Enterobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.06
-
trimethylpyruvate pH 8.0, 37°C Enterobacter sp.
2.6.1.42 0.09
-
trimethylpyruvate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.42
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 0.63
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 0.78
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 0.97
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 0.99
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 1.64
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 7.38
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Escherichia coli
2.6.1.42 9.52
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Enterobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Enterobacter sp.
-
-
-
2.6.1.42 Enterobacter sp. TL3
-
-
-
2.6.1.42 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 immobilized metal ion affinity chromatography (Ni2+) Escherichia coli
2.6.1.42 immobilized metal ion affinity chromatography (Ni2+) Enterobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate
-
Escherichia coli 3-hydroxyadamantylglycine + 2-oxoglutarate
-
?
2.6.1.42 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate
-
Enterobacter sp. 3-hydroxyadamantylglycine + 2-oxoglutarate
-
?
2.6.1.42 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + L-glutamate
-
Enterobacter sp. TL3 3-hydroxyadamantylglycine + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxobutyric acid + L-glutamate
-
Escherichia coli L-valine + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxobutyric acid + L-glutamate
-
Enterobacter sp. L-valine + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxobutyric acid + L-glutamate
-
Enterobacter sp. TL3 L-valine + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxovaleric acid + L-glutamate
-
Escherichia coli L-isoleucine + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. L-isoleucine + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. TL3 L-isoleucine + 2-oxoglutarate
-
?
2.6.1.42 4-methyl-2-oxovaleric acid + L-glutamate
-
Escherichia coli L-leucine + 2-oxoglutarate
-
?
2.6.1.42 4-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. L-leucine + 2-oxoglutarate
-
?
2.6.1.42 4-methyl-2-oxovaleric acid + L-glutamate
-
Enterobacter sp. TL3 L-leucine + 2-oxoglutarate
-
?
2.6.1.42 trimethylpyruvate + L-glutamate
-
Escherichia coli L-tert-leucine + 2-oxoglutarate
-
?
2.6.1.42 trimethylpyruvate + L-glutamate
-
Enterobacter sp. L-tert-leucine + 2-oxoglutarate
-
?
2.6.1.42 trimethylpyruvate + L-glutamate
-
Enterobacter sp. TL3 L-tert-leucine + 2-oxoglutarate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 branched chain aminotransferase
-
Escherichia coli
2.6.1.42 branched chain aminotransferase
-
Enterobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.42 2.2
-
trimethylpyruvate pH 8.0, 37°C Escherichia coli
2.6.1.42 2.59
-
trimethylpyruvate pH 8.0, 37°C Enterobacter sp.
2.6.1.42 2.9
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 4.11
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Escherichia coli
2.6.1.42 6.61
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 12.15
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 12.45
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 13.75
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 16.84
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 17.32
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.42 7.5
-
-
Escherichia coli
2.6.1.42 7.5
-
-
Enterobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.42 6 9 58% activity at pH 6.0, 78% activity at pH 9.0 Escherichia coli
2.6.1.42 6 9 58% activity at pH 6.0, 78% activity at pH 9.0 Enterobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.42 0.3
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 0.56
-
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid pH 8.0, 37°C Escherichia coli
2.6.1.42 10.27
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 13.89
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 15.74
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Escherichia coli
2.6.1.42 15.96
-
3-methyl-2-oxobutyric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 17.85
-
4-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 19.29
-
3-methyl-2-oxovaleric acid pH 8.0, 37°C Enterobacter sp.
2.6.1.42 24.44
-
trimethylpyruvate pH 8.0, 37°C Escherichia coli
2.6.1.42 43.16
-
trimethylpyruvate pH 8.0, 37°C Enterobacter sp.