Literature summary extracted from

  • Kato, M.
    Trehalose production with a new enzymatic system from Sulfolobus solfataricus KM1 (1999), J. Mol. Catal. B, 6, 223-233.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.141 synthesis trehalose production from starch Saccharolobus solfataricus
5.4.99.15 synthesis trehalose production from starch Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.141 expression in Escherichia coli Saccharolobus solfataricus
5.4.99.15 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.15 1.2
-
maltoheptaose pH 5.5, 60°C Saccharolobus solfataricus
3.2.1.141 5.8
-
maltosyltriosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus
3.2.1.141 4.7
-
maltosylpentaosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus
5.4.99.15 2.7
-
maltohexaose pH 5.5, 60°C Saccharolobus solfataricus
5.4.99.15 5
-
maltopentaose pH 5.5, 60°C Saccharolobus solfataricus
3.2.1.141 5.6
-
maltosyltetraosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus
5.4.99.15 21.8
-
maltotetraose pH 5.5, 60°C Saccharolobus solfataricus
5.4.99.15 228
-
maltotriose pH 5.5, 60°C Saccharolobus solfataricus
3.2.1.141 22.4
-
maltosyltrehalose pH 5.5, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.141 65000
-
x * 65000, calculated from sequence Saccharolobus solfataricus
3.2.1.141 61000
-
x * 61000, SDS-PAGE Saccharolobus solfataricus
5.4.99.15 76000
-
x * 76000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.15 Saccharolobus solfataricus
-
-
-
3.2.1.141 Saccharolobus solfataricus Q55088
-
-
5.4.99.15 Saccharolobus solfataricus KM1
-
-
-
3.2.1.141 Saccharolobus solfataricus KM1 Q55088
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 additional information no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products Saccharolobus solfataricus ?
-
?
5.4.99.15 additional information no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products Saccharolobus solfataricus KM1 ?
-
?
5.4.99.15 maltoheptaose
-
Saccharolobus solfataricus alpha-maltopentaosyl trehalose
-
?
5.4.99.15 maltoheptaose
-
Saccharolobus solfataricus KM1 alpha-maltopentaosyl trehalose
-
?
5.4.99.15 maltohexaose
-
Saccharolobus solfataricus alpha-maltotetraosyltrehalose
-
?
5.4.99.15 maltohexaose
-
Saccharolobus solfataricus KM1 alpha-maltotetraosyltrehalose
-
?
5.4.99.15 maltopentaose
-
Saccharolobus solfataricus alpha-maltotriosyltrehalose
-
?
5.4.99.15 maltopentaose
-
Saccharolobus solfataricus KM1 alpha-maltotriosyltrehalose
-
?
3.2.1.141 maltosylpentaosyltrehalose + H2O 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltopentaose
-
?
3.2.1.141 maltosylpentaosyltrehalose + H2O 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltopentaose
-
?
3.2.1.141 maltosyltetraosyltrehalose + H2O hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltotetraose
-
?
3.2.1.141 maltosyltetraosyltrehalose + H2O hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltotetraose
-
?
3.2.1.141 maltosyltrehalose + H2O 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltose
-
?
3.2.1.141 maltosyltrehalose + H2O 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltose
-
?
3.2.1.141 maltosyltriosyltrehalose + H2O 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltotriose
-
?
3.2.1.141 maltosyltriosyltrehalose + H2O 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltotriose
-
?
5.4.99.15 maltotetraose
-
Saccharolobus solfataricus alpha-maltosyltrehalose
-
?
5.4.99.15 maltotetraose
-
Saccharolobus solfataricus KM1 alpha-maltosyltrehalose
-
?
5.4.99.15 maltotriose
-
Saccharolobus solfataricus alpha-glucosyltrehalose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.141 ? x * 61000, SDS-PAGE Saccharolobus solfataricus
3.2.1.141 ? x * 65000, calculated from sequence Saccharolobus solfataricus
5.4.99.15 ? x * 76000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.141 70 85
-
Saccharolobus solfataricus
5.4.99.15 70 80
-
Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.141 85
-
6 h, stable Saccharolobus solfataricus
5.4.99.15 85
-
6 h, 9% loss of activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.141 4.5 5.5
-
Saccharolobus solfataricus
5.4.99.15 5 6
-
Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.15 4 10 stable Saccharolobus solfataricus
3.2.1.141 3.5 10 stable Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.141 Saccharolobus solfataricus isoelectric focusing
-
4.8
5.4.99.15 Saccharolobus solfataricus isoelectric focusing
-
6.1