EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.141 | synthesis | trehalose production from starch | Saccharolobus solfataricus |
5.4.99.15 | synthesis | trehalose production from starch | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.141 | expression in Escherichia coli | Saccharolobus solfataricus |
5.4.99.15 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.141 | 4.7 | - |
maltosylpentaosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
3.2.1.141 | 5.6 | - |
maltosyltetraosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
3.2.1.141 | 5.8 | - |
maltosyltriosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
3.2.1.141 | 22.4 | - |
maltosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.4.99.15 | 1.2 | - |
maltoheptaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.4.99.15 | 2.7 | - |
maltohexaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.4.99.15 | 5 | - |
maltopentaose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.4.99.15 | 21.8 | - |
maltotetraose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.4.99.15 | 228 | - |
maltotriose | pH 5.5, 60°C | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 61000 | - |
x * 61000, SDS-PAGE | Saccharolobus solfataricus |
3.2.1.141 | 65000 | - |
x * 65000, calculated from sequence | Saccharolobus solfataricus |
5.4.99.15 | 76000 | - |
x * 76000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.141 | Saccharolobus solfataricus | Q55088 | - |
- |
3.2.1.141 | Saccharolobus solfataricus KM1 | Q55088 | - |
- |
5.4.99.15 | Saccharolobus solfataricus | - |
- |
- |
5.4.99.15 | Saccharolobus solfataricus KM1 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.141 | maltosylpentaosyltrehalose + H2O | 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltopentaose | - |
? | |
3.2.1.141 | maltosylpentaosyltrehalose + H2O | 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltopentaose | - |
? | |
3.2.1.141 | maltosyltetraosyltrehalose + H2O | hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltotetraose | - |
? | |
3.2.1.141 | maltosyltetraosyltrehalose + H2O | hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltotetraose | - |
? | |
3.2.1.141 | maltosyltrehalose + H2O | 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltose | - |
? | |
3.2.1.141 | maltosyltrehalose + H2O | 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltose | - |
? | |
3.2.1.141 | maltosyltriosyltrehalose + H2O | 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltotriose | - |
? | |
3.2.1.141 | maltosyltriosyltrehalose + H2O | 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltotriose | - |
? | |
5.4.99.15 | maltoheptaose | - |
Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | - |
? | |
5.4.99.15 | maltoheptaose | - |
Saccharolobus solfataricus KM1 | alpha-maltopentaosyl trehalose | - |
? | |
5.4.99.15 | maltohexaose | - |
Saccharolobus solfataricus | alpha-maltotetraosyltrehalose | - |
? | |
5.4.99.15 | maltohexaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotetraosyltrehalose | - |
? | |
5.4.99.15 | maltopentaose | - |
Saccharolobus solfataricus | alpha-maltotriosyltrehalose | - |
? | |
5.4.99.15 | maltopentaose | - |
Saccharolobus solfataricus KM1 | alpha-maltotriosyltrehalose | - |
? | |
5.4.99.15 | maltotetraose | - |
Saccharolobus solfataricus | alpha-maltosyltrehalose | - |
? | |
5.4.99.15 | maltotetraose | - |
Saccharolobus solfataricus KM1 | alpha-maltosyltrehalose | - |
? | |
5.4.99.15 | maltotriose | - |
Saccharolobus solfataricus | alpha-glucosyltrehalose | - |
? | |
5.4.99.15 | additional information | no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products | Saccharolobus solfataricus | ? | - |
? | |
5.4.99.15 | additional information | no activity with maltose. The enzyme never produces a glycosyltrehalose of longer chain length than the substrate used. Glucose and maltooligosaccharide shortened by one glucose residue are produced as by-products | Saccharolobus solfataricus KM1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.141 | ? | x * 61000, SDS-PAGE | Saccharolobus solfataricus |
3.2.1.141 | ? | x * 65000, calculated from sequence | Saccharolobus solfataricus |
5.4.99.15 | ? | x * 76000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 70 | 85 | - |
Saccharolobus solfataricus |
5.4.99.15 | 70 | 80 | - |
Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 85 | - |
6 h, stable | Saccharolobus solfataricus |
5.4.99.15 | 85 | - |
6 h, 9% loss of activity | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 4.5 | 5.5 | - |
Saccharolobus solfataricus |
5.4.99.15 | 5 | 6 | - |
Saccharolobus solfataricus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.141 | 3.5 | 10 | stable | Saccharolobus solfataricus |
5.4.99.15 | 4 | 10 | stable | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.141 | Saccharolobus solfataricus | isoelectric focusing | - |
4.8 |
5.4.99.15 | Saccharolobus solfataricus | isoelectric focusing | - |
6.1 |