EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.102 | mutant enzymes are expressed in HEK-293T cells | Mus musculus |
2.4.1.148 | mutant enzyme C217S is expressed in HEK-293T cells | Mus musculus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.102 | C217S mutant in complex with UDP, hanging drop vapor diffusion method, using 24% (w/v) polyethylene glycol 4000, 0.1 M glycine (pH 9.0) and 0.6 M LiCl | Mus musculus |
2.4.1.148 | mutant enzyme C217S in complex with UDP, hanging drop vapor diffusion method, using 24% (w/v) polyethylene glycol 4000, 0.1 M glycine (pH 9.0) and 0.6 M LiCl | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.102 | C217S | the mutant is catalytically active | Mus musculus |
2.4.1.102 | K401A/C217S | inactive | Mus musculus |
2.4.1.102 | R378A/C217S | inactive | Mus musculus |
2.4.1.102 | R378A/K401A/C217S | inactive | Mus musculus |
2.4.1.148 | C217S | the mutant is not oxidatively inactivated, and its kinetic parameters are very similar to those of the native enzyme | Mus musculus |
2.4.1.148 | K401A/C217S | catalytically inactive | Mus musculus |
2.4.1.148 | R378A/C217S | catalytically inactive | Mus musculus |
2.4.1.148 | R378A/K401A/C217S | catalytically inactive | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.102 | UDP | - |
Mus musculus | |
2.4.1.102 | UMP | - |
Mus musculus | |
2.4.1.102 | uridine | - |
Mus musculus | |
2.4.1.148 | UDP | - |
Mus musculus | |
2.4.1.148 | UMP | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.102 | 2.8 | - |
UDP-N-acetyl-D-glucosamine | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
2.4.1.148 | 2.8 | - |
UDP-N-acetyl-D-glucosamine | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.148 | additional information | metal-ion-independent enzyme | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.148 | UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-Ser/Thr | Mus musculus | - |
UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-alpha-D-galactosaminyl-Ser/Thr | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.102 | Mus musculus | Q09324 | - |
- |
2.4.1.148 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.102 | IgG-Sepharose bead chromatography | Mus musculus |
2.4.1.148 | IgG-Sepahrose beads chromatography, gel filtration | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.102 | leukocyte | - |
Mus musculus | - |
2.4.1.148 | leukocyte | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.148 | 3 | - |
mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.102 | UDP-N-acetyl-D-glucosamine + Galbeta1-3GalNAcalpha-4-nitrophenyl | - |
Mus musculus | ? | - |
? | |
2.4.1.148 | UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-Ser/Thr | - |
Mus musculus | UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-alpha-D-galactosaminyl-Ser/Thr | - |
? | |
2.4.1.148 | UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosaminyl-4-nitrophenyl | - |
Mus musculus | UDP + N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,3)-N-acetyl-D-galactosaminyl-4-nitrophenyl | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.102 | C2GnT-I | - |
Mus musculus |
2.4.1.102 | C2GnT-L | - |
Mus musculus |
2.4.1.102 | core 2 beta1,6-N-acetylglucosaminyltransferase | - |
Mus musculus |
2.4.1.148 | C2GnT-I | - |
Mus musculus |
2.4.1.148 | C2GnT-L | - |
Mus musculus |
2.4.1.148 | leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase | - |
Mus musculus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.102 | 0.23 | - |
UMP | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
2.4.1.102 | 0.26 | - |
UDP | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
2.4.1.148 | 0.23 | - |
UMP | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus | |
2.4.1.148 | 0.26 | - |
UDP | mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C | Mus musculus |