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Literature summary extracted from

  • Pak, J.E.; Satkunarajah, M.; Seetharaman, J.; Rini, J.M.
    Structural and mechanistic characterization of leukocyte-type core 2 ?1,6-N-acetylglucosaminyltransferase: a metal-ion-independent GT-A glycosyltransferase (2011), J. Mol. Biol., 414, 798-811.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.102 mutant enzymes are expressed in HEK-293T cells Mus musculus
2.4.1.148 mutant enzyme C217S is expressed in HEK-293T cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.102 C217S mutant in complex with UDP, hanging drop vapor diffusion method, using 24% (w/v) polyethylene glycol 4000, 0.1 M glycine (pH 9.0) and 0.6 M LiCl Mus musculus
2.4.1.148 mutant enzyme C217S in complex with UDP, hanging drop vapor diffusion method, using 24% (w/v) polyethylene glycol 4000, 0.1 M glycine (pH 9.0) and 0.6 M LiCl Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.102 C217S the mutant is catalytically active Mus musculus
2.4.1.102 K401A/C217S inactive Mus musculus
2.4.1.102 R378A/C217S inactive Mus musculus
2.4.1.102 R378A/K401A/C217S inactive Mus musculus
2.4.1.148 C217S the mutant is not oxidatively inactivated, and its kinetic parameters are very similar to those of the native enzyme Mus musculus
2.4.1.148 K401A/C217S catalytically inactive Mus musculus
2.4.1.148 R378A/C217S catalytically inactive Mus musculus
2.4.1.148 R378A/K401A/C217S catalytically inactive Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.102 UDP
-
Mus musculus
2.4.1.102 UMP
-
Mus musculus
2.4.1.102 uridine
-
Mus musculus
2.4.1.148 UDP
-
Mus musculus
2.4.1.148 UMP
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.102 2.8
-
UDP-N-acetyl-D-glucosamine mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus
2.4.1.148 2.8
-
UDP-N-acetyl-D-glucosamine mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.148 additional information metal-ion-independent enzyme Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.148 UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-Ser/Thr Mus musculus
-
UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Mus musculus Q09324
-
-
2.4.1.148 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.102 IgG-Sepharose bead chromatography Mus musculus
2.4.1.148 IgG-Sepahrose beads chromatography, gel filtration Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.102 leukocyte
-
Mus musculus
-
2.4.1.148 leukocyte
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.148 3
-
mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 UDP-N-acetyl-D-glucosamine + Galbeta1-3GalNAcalpha-4-nitrophenyl
-
Mus musculus ?
-
?
2.4.1.148 UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
-
Mus musculus UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
-
?
2.4.1.148 UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosaminyl-4-nitrophenyl
-
Mus musculus UDP + N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,3)-N-acetyl-D-galactosaminyl-4-nitrophenyl
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.102 C2GnT-I
-
Mus musculus
2.4.1.102 C2GnT-L
-
Mus musculus
2.4.1.102 core 2 beta1,6-N-acetylglucosaminyltransferase
-
Mus musculus
2.4.1.148 C2GnT-I
-
Mus musculus
2.4.1.148 C2GnT-L
-
Mus musculus
2.4.1.148 leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase
-
Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.102 0.23
-
UMP mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus
2.4.1.102 0.26
-
UDP mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus
2.4.1.148 0.23
-
UMP mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus
2.4.1.148 0.26
-
UDP mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus