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Literature summary extracted from

  • Chetnani, B.; Kumar, P.; Surolia, A.; Vijayan, M.
    M. tuberculosis pantothenate kinase: dual substrate specificity and unusual changes in ligand locations (2010), J. Mol. Biol., 400, 171-185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 complexes of the enzyme with guanosine-5'-[(beta,gamma)-methyleno]triphosphate and pantothenate, GDP and phosphopantothenate, GDP, GDP and pantothenate, 5'-adenylyl (beta,gamma-methylene)diphosphonate, and guanosine-5'-[(beta,gamma)-methyleno]triphosphate, hanging drop vapor diffusion method, using 1.4-1.8 M trisodium citrate, 0.05-0.1 M sodium acetate, and 10% (v/v) glycerol at pH 6.5 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.616
-
 ATP in citrate buffer, pH 6.5, temperature not specified in the publication Mycobacterium tuberculosis
2.7.1.33 668
-
 GTP in citrate buffer, pH 6.5, temperature not specified in the publication Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Mycobacterium tuberculosis ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 GTP + (R)-pantothenate
-
 Mycobacterium tuberculosis GDP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 0.052
-
 ATP in citrate buffer, pH 6.5, temperature not specified in the publication Mycobacterium tuberculosis
2.7.1.33 0.064
-
 GTP in citrate buffer, pH 6.5, temperature not specified in the publication Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.33 0.084
-
 ATP in citrate buffer, pH 6.5, temperature not specified in the publication Mycobacterium tuberculosis
2.7.1.33 0.095
-
 GTP in citrate buffer, pH 6.5, temperature not specified in the publication Mycobacterium tuberculosis