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Literature summary extracted from

  • Rea, D.; Fulop, V.; Bugg, T.D.; Roper, D.I.
    Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli (2007), J. Mol. Biol., 373, 866-876.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.52 HpcH gene expressed in Escherichia coli strain B834 (DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.52 crystallization of apo-HpcH, because divalent metal ion is lost during the purification process, resulting in preparation of the inactive apo form of the enzyme, crystals grown using the hanging-drop vapour diffusion method Escherichia coli
4.1.2.52 HpcH–Mg2+–oxamate crystals grown with the addition of 10 mM magnesium chloride and sodium oxamate (substrate analogue), elucidation of active site architecture Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.52 28000
-
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.52 4-hydroxy-2-oxo-heptanedioate Escherichia coli
-
pyruvate + succinic semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.52 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.52 metal affinity and size exclusion chromatography, Superdex 200 column used Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.52 4-hydroxy-2-oxo-heptanedioate
-
Escherichia coli pyruvate + succinic semialdehyde
-
?

Subunits

EC Number Subunits Comment Organism
4.1.2.52 hexamer 6 * 28000 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.1.2.52 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
-
Escherichia coli
4.1.2.52 HpcH
-
Escherichia coli

General Information

EC Number General Information Comment Organism
4.1.2.52 metabolism enzyme is part of homoprotocatechuate degradation pathway Escherichia coli