EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.B3 | - |
Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.1.1.B3 | determination of strucure by molecular replacement, to 1.5 A resolution. Enzyme possesses a Zn2+ located in the putative tRNA acceptor stem-binding domain. The Zn2+ ion is coordinated by three cysteine and a tyrosine ligand | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.B3 | glutamol-adenosine monophosphate | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.B3 | 0.5 | - |
ATP | pH 7.2, 37°C, activation of L-glutamate | Escherichia coli | |
6.1.1.B3 | 6 | - |
L-glutamate | pH 7.2, 37°C, activation of L-glutamate | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.B3 | Zn2+ | enzyme possesses a Zn2+ ion located in the putative tRNA acceptor stem-binding domain. The Zn2+ ion is coordinated by three cysteine and a tyrosine ligand | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.B3 | Escherichia coli | P27305 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.B3 | ATP + L-glutamate + tRNA | acceptor is unfractionated RNA | Escherichia coli | AMP + diphosphate + L-glutamyl-tRNA | the activation step of L-glutamate is tRNA-independent | ? | |
6.1.1.B3 | additional information | enzyme does not catalyse attachment of the activated L-glutamate to Escherichia coli tRNAGlu | Escherichia coli | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.B3 | 14 | - |
L-glutamate | pH 7.2, 37°C, activation of L-glutamate | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.B3 | 0.2 | - |
glutamol-adenosine monophosphate | pH 7.2, 37°C, substrate ATP, activation of L-glutamate | Escherichia coli | |
6.1.1.B3 | 0.44 | - |
glutamol-adenosine monophosphate | pH 7.2, 37°C, substrate L-glutamate, activation of L-glutamate | Escherichia coli |