BRENDA - Enzyme Database

Kinetics of a cloned special ginsenosidase hydrolyzing 3-O-glucoside of multi-protopanaxadiol-type ginsenosides, named ginsenosidase type III

Jin, X.F.; Yu, H.S.; Wang, D.M.; Liu, T.Q.; Liu, C.Y.; An, D.S.; Im, W.T.; Kim, S.G.; Jin, F.X.; J. Microbiol. Biotechnol. 22, 343-351 (2012) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.191
expression in Escherichia coli
Terrabacter ginsenosidimutans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.191
3.85
-
ginsenoside Rc
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
4.08
-
ginsenoside Rb1
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
8.85
-
ginsenoside Rb3
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
9.09
-
ginsenoside Rb2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
9.7
-
ginsenoside Rg3(S)
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
11.4
-
ginsenoside Rd
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
12.9
-
ginsenoside F2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.191
71140
-
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
3.2.1.191
72000
-
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.191
Terrabacter ginsenosidimutans
D2KAT1
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.191
-
Terrabacter ginsenosidimutans
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.2.1.191
a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + 2 H2O = a protopanaxadiol-type ginsenoside with no glycosidic modification at position 3 + 2 D-glucopyranose
overall reaction
Terrabacter ginsenosidimutans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.191
ginsenoside C-Mc1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside C-Mx1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside C-O + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Y + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside F2 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-K + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rb1 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside XVII + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rb2 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
ginsenoside C-O + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rb3 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx1 + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rc + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc1 + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rd + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside F2 + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rg3(S) + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside Rh2(S) + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rh2(S) + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
protopanaxadiol aglycone + D-glucopyranose
-
-
-
?
3.2.1.191
gypenoside XVII + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside LXXV + D-glucopyranose
-
-
-
?
3.2.1.191
additional information
the enzyme is able to hydrolyse 3-O-glucoside of multi-protopanaxadiol-type ginsenosides. Similarly, the enzyme can hydrolyse the glucopyranosyls linked to the 3-O-position of Rb2, Rc, Rd, Rb3, and Rg3
722936
Terrabacter ginsenosidimutans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.191
?
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
3.2.1.191
?
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.191
3-O-multi-glycoside ginsenosidase
-
Terrabacter ginsenosidimutans
3.2.1.191
bgpA
-
Terrabacter ginsenosidimutans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.191
40
-
assay at
Terrabacter ginsenosidimutans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.191
7
-
assay at
Terrabacter ginsenosidimutans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.191
expression in Escherichia coli
Terrabacter ginsenosidimutans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.191
3.85
-
ginsenoside Rc
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
4.08
-
ginsenoside Rb1
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
8.85
-
ginsenoside Rb3
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
9.09
-
ginsenoside Rb2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
9.7
-
ginsenoside Rg3(S)
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
11.4
-
ginsenoside Rd
pH 7.0, 40°C
Terrabacter ginsenosidimutans
3.2.1.191
12.9
-
ginsenoside F2
pH 7.0, 40°C
Terrabacter ginsenosidimutans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.191
71140
-
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
3.2.1.191
72000
-
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.191
-
Terrabacter ginsenosidimutans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.191
ginsenoside C-Mc1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside C-Mx1 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside C-O + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Y + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside F2 + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-K + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rb1 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside XVII + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rb2 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl
722936
Terrabacter ginsenosidimutans
ginsenoside C-O + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rb3 + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mx1 + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rc + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside C-Mc1 + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rd + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside F2 + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rg3(S) + H2O
hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
ginsenoside Rh2(S) + D-glucopyranose
-
-
-
?
3.2.1.191
ginsenoside Rh2(S) + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl bond
722936
Terrabacter ginsenosidimutans
protopanaxadiol aglycone + D-glucopyranose
-
-
-
?
3.2.1.191
gypenoside XVII + H2O
hydrolysis of 3-O-beta-D-glucopyranosyl
722936
Terrabacter ginsenosidimutans
gypenoside LXXV + D-glucopyranose
-
-
-
?
3.2.1.191
additional information
the enzyme is able to hydrolyse 3-O-glucoside of multi-protopanaxadiol-type ginsenosides. Similarly, the enzyme can hydrolyse the glucopyranosyls linked to the 3-O-position of Rb2, Rc, Rd, Rb3, and Rg3
722936
Terrabacter ginsenosidimutans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.191
?
x * 72000, SDS-PAGE
Terrabacter ginsenosidimutans
3.2.1.191
?
x * 71140, calculated from sequence
Terrabacter ginsenosidimutans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.191
40
-
assay at
Terrabacter ginsenosidimutans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.191
7
-
assay at
Terrabacter ginsenosidimutans