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Literature summary extracted from

  • Rambhatla, P.; Kumar, S.; Floyd, J.T.; Varela, M.F.
    Molecular cloning and characterization of mannitol-1-phosphate dehydrogenase from Vibrio cholerae (2011), J. Microbiol. Biotechnol., 21, 914-920.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 gene mtld1, encoded in the mannitol operon, sequence comparison, real-time PCR expression analysis, expression as His6-tagged protein in Escherichia coli strain SG13009 Vibrio cholerae serotype O1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.17 additional information 5 and 10 mM concentrations of MgSO4, MnSO4, FeCl3, ZnCl2, CoCl2, and LiCl have no effect on the enzyme activity Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Vibrio cholerae serotype O1
-
 D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Vibrio cholerae serotype O1
-
 gene mtlD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 recombinant His6-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography Vibrio cholerae serotype O1

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 additional information gene mtlD is constitutively expressed even when no mannitol wis present in the growth medium Vibrio cholerae serotype O1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 3.32
-
 recombinant enzyme, crude extract, pH 7.5, 30°C, fructose 6-phosphate reduction Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Vibrio cholerae serotype O1 D-fructose 6-phosphate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 mannitol-1-phosphate dehydrogenase
-
 Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 30
-
 assay at Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 7.5
-
 assay at, reduction reaction Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+ the NAD+-binding consensus sequence GxGxxGxGxxG is localized within the highly conserved N-terminal portion of MtlD corresponding to the amino acid residues 9-19 Vibrio cholerae serotype O1
1.1.1.17 NADH
-
 Vibrio cholerae serotype O1

General Information

EC Number General Information Comment Organism
1.1.1.17 evolution the enzyme belongs to the medium chain dehydrogenases Vibrio cholerae serotype O1